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International Journal of Nephrology
Volume 2012, Article ID 284896, 10 pages
http://dx.doi.org/10.1155/2012/284896
Research Article

Vitamin E, Vitamin C, or Losartan Is Not Nephroprotectant against Cisplatin-Induced Nephrotoxicity in Presence of Estrogen in Ovariectomized Rat Model

1Water and Electrolytes Research Center, Isfahan University of Medical Sciences, HezarJerib Avenue, Isfahan 81745, Iran
2Department of Physiology, Isfahan University of Medical Sciences, HezarJerib Avenue, Isfahan 81745, Iran
3Kidney Diseases Research Center, Isfahan University of Medical Sciences, HezarJerib Avenue, Isfahan 81745, Iran
4Department of Internal Medicine, Isfahan University of Medical Sciences, HezarJerib Avenue, Isfahan 81745, Iran
5Department of Clinical Pathology, Isfahan University of Medical Sciences, HezarJerib Avenue, Isfahan 81745, Iran

Received 12 June 2012; Revised 14 August 2012; Accepted 20 August 2012

Academic Editor: James E. Springate

Copyright © 2012 Mehdi Nematbakhsh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The nephroprotective effect of vitamins E and C or losartan against cisplatin (CP)- induced nephrotoxicity when they are accompanied by estrogen was investigated. Methods. The ovariectomized rats received estradiol valerate for two weeks. At the end of the first week, a single dose of CP (7 mg/kg, IP) was also administered, and they received placebo (group 1), vitamin E (group 2), vitamin C (group 3), or losartan (group 4) every day during the second week, and they were compared with another three control groups. Results. CP alone increased the serum levels of blood urea nitrogen (BUN), creatinine (Cr), and kidney tissue damage score (KTDS), significantly ( ), however at the presence of estradiol and CP, vitamin C, vitamin E, or losartan not only did not decrease these parameters, but also increased them significantly ( ). The serum level of superoxidase dismutase (SOD) was reduced by CP ( ), but it was increased when estradiol or estradiol plus vitamin C or losartan were added ( ). Conclusion. The particular pharmacological dose of estrogen used in this study abolish the nephroprotective effects vitamins C and E or losartan against CP-induced nephrotoxicity.