Classification of Five Uremic Solutes according to Their Effects on Renal Tubular Cells

Figure 6

Effects of uremic solutes on p53 and Chk1 phosphorylation. Porcine renal tubular cells were treated with each uremic solute for 5 hours. Cell lysates were obtained and subjected to Western blotting. Cont, control; IS, indoxyl sulfate; PCS, p-cresyl sulfate; PhS, phenyl sulfate; HA, hippuric acid; IAA, indoleacetic acid. Images are representative of two independent experiments.