Classification of Five Uremic Solutes according to Their Effects on Renal Tubular Cells

Table 1

Primers for real-time PCR.

Target	Regulation	Forward	Reverse
p21	p53-regulated	5′-catgtggacctgttgctgtc-3′	5′-cctcttggagaagatcagcc-3′
ATF4	ER stress responsive	5′-ggtcagtgcctcagacaaca-3′	5′-tctggcatggtttccaggtc-3′
CHOP	ER stress responsive	5′-cttcaccactcttgaccctg-3′	5′-gagctctgactggaatcagg-3′
Cdc20	p53-regulated	5′-aatgtcctggcaactggagg-3′	5′-tgagctccttgtagtgggga-3′
Skp2	p53-regulated	5′-acctccacccagatgtgact-3′	5′-gtgctgtacacgaaaagggc-3′
Puma	p53-regulated	5′-gatctcaacgcgctgtacga-3′	5′-caggcacctaattaggctcc-3′
GAPDH	house-keeping gene	5′-ccatcaccatcttccaggag-3′	5′-gagatgatgaccctcttggc-3′