Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β

Figure 2

Meprin B degrades purified recombinant human OS-9. Purified recombinant OS-9 was incubated with activated forms of homomeric meprin A and meprin B for 0–4 h. Western blot analysis was used to evaluate for degradation or fragmentation of OS-9. The levels of OS-9 decreased in a time-dependent manner in reactions with activated meprin B, but not in reactions with activated meprin A or Tris buffer controls without meprins.