Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis

Figure 2

(a) Representative blot shows cleaved caspase-3 protein expression is increased in mouse RTECs treated with 10 µM and 50 µM cisplatin compared to control mouse RTECs, control squirrel RTECs, and squirrel RTECs treated with 10 µM and 50 µM cisplatin. (b) TUNEL + apoptotic cells are significantly increased in mouse RTECs treated with 10 µM and 50 µM cisplatin versus control mouse RTECs, control squirrel RTECs, and cisplatin-treated squirrel RTECs ( versus mouse and squirrel RTECs treated with 0 µM cisplatin (controls) and squirrel RTECs treated with 10 µM and 50 µM cisplatin, n = 3). (c) Representative pictures of TUNEL staining in squirrel and mouse RTECs treated with cisplatin. β-actin is used as a protein loading control. Vehicle treated cells are referred to as controls. A densitometry and statistical analysis of immunoblot is given in Supplementary Figure S1.

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