Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis

Figure 5

(a) Representative blots show reduced expression of XIAP in squirrel RTECs treated with XIAP siRNA. Cisplatin and XIAP siRNA-treated squirrel RTECs have decreased protein expression of XIAP and increased expression of cleaved caspase-3 compared to controls and wild type cisplatin-treated squirrel RTECs. (b) TUNEL + apoptotic cells are increased significantly in 50 µM cisplatin-treated XIAP deficient squirrel RTECs versus wild type and XIAP deficient controls and wild type cisplatin-treated squirrel RTECs (versus wild type and XIAP deficient squirrel RTECs treated with 0 µM cisplatin (controls), wild type squirrel RTECs treated with 10 µM and 50 µM cisplatin, and XIAP deficient squirrel RTECs treated with 10 µM cisplatin, n = 3). (c) Representative pictures of TUNEL staining in wild type and XIAP negative squirrel RTECs treated with cisplatin. β-actin is used as a protein loading control. Vehicle treated cells are referred to as controls. Densitometry and statistical analyses of immunoblots are given in Supplementary Figure S4.

(a)

(b)

(c)