Bevacizumab Increases Endothelin-1 Production via Forkhead Box Protein O1 in Human Glomerular Microvascular Endothelial Cells In Vitro

Figure 1

Bevacizumab induced ET-1 production in cultured human glomerular microvascular endothelial cells (hGECs). hGECs were plated in 24-well plates (1 × 10⁵ cells/well) for ELISA or 60 mm culture dishes (1 × 10⁶ cells/dish) for RT-PCR. hGECs that were 90% confluent were serum-starved for 24 h before the experiments were performed. hGECs were treated with 0.1 or 1 µM bevacizumab for 8 h, RNA was extracted from the cells, and ET-1 mRNA level was examined by real-time RT-PCR (a). The medium was collected and ET-1 protein level was examined by the ELISA (b). Data shown are means ± SD (n = 3;  < 0.05). The experiments were repeated at least three times, with reproducible results.

(a)

(b)