PAC-Mediated AKI Protection Is Critically Mediated but Does Not Exclusively Depend on Cell-Derived Microvesicles

Figure 6

Protein interaction networks between the proteins found to be differentially secreted upon treatments were generated using the protein networks software String (https://string-db.org). (a) Interaction network of the secretome proteins that were more than twofold secreted in Ang-2 treated cells compared to controls (ratio Agn-2/Ctr > 2). (b) Interaction network of proteins found to be downregulated (ratio Agn-2/Ctr < 0.5) under Ang-2 treatment compared to control. Interestingly, the interaction network showed that Ang-2 treatment resulted in decreased secretion of proteins involved in the coagulation cascade and in platelet activation (red and blue colored). (c) Interaction network of proteins, which level was downregulated (ratio BMP5/Ctr < 0.5) in secretome of BMP5 treated cells. (d) Interaction network of proteins highly secreted upon Ang-2 treatment compared to BMP5 treatment (ratio Ang-2/BMP5 >2). The network revealed that Ang-2 treatment resulted in significant alteration of EPC secretome.

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