Diagnostic Accuracy of Direct Immunofluorescence Test on Paraffin-Embedded Blocks in Comparison with Frozen Section Blocks in Renal Biopsies

Table 1

Antibodies used immunofluorescence on formalin-fixed, paraffin-embedded tissue sections and immunofluorescence on frozen sections staining methods.

Staining methods	Antibodies	Antibody (g/L)	Dilution	Incubation time (min)
IF-F	IgA, FTTC	1.5	1 : 40	45
	IgG, FTTC	1.1	1 : 40	45
	IgM, FTTC	4.1	1 : 40	45
	C3, FITC	1.1	1 : 40	45
IF-P	IgA, FTTC	1.5	1 : 40	60
	IgG, FTTC	1.1	1 : 40	60
	IgM, FTTC	4.1	1 : 40	60
	C3, FITC	1.1	1 : 40	60

FTTC: Fluorescein isothiocyanate; IF-F: Immunofluorescence on frozen sections; IF-P: Immunofluorescence on formalin-fixed, paraffin-embedded tissue sections. All antibodies were obtained from Dako (Glostrup, Denmark). All antibodies were diluted with antibody diluent (code no. S0809: Dako)