Bayesian Estimation of the Axial Position in Astigmatism-Based Three-Dimensional Particle Tracking

Figure 2

Calibration probability distributions. (a) 2D plots of 𝑝(𝜎_𝑥,𝜎_𝑦∣𝑧) at three different axial positions. (b) Total probability function 𝑝(𝜎_𝑥,𝜎_𝑦) of measuring a pair of widths (𝜎_𝑥,𝜎_𝑦). The lateral size of each image is 5 pixels.

(a)

(b)