Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber

Figure 1

(a) Schematic of the setup: two computer controlled actuators adjust the position and orientation of a side-firing optical fiber inside the tissue. A fiber rotary joint is used to help rotate the fiber with no mechanical stress. At each position, the system sends an excitation laser pulse and detects a sample of the corresponding emission signal. Next, the collected data is processed by a computer to generate images that show the distribution of fluorescence signal in a cylinder surrounding the fiber; (b) picture of the developed system.