Mechanisms of the influence of UV irradiation on collagen and collagen-ascorbic acid solutions
The study of the influence of UV irradiation on collagen solutions has shown the destabilization of the collagen molecule by calorimetric method. It is reflected both in changes of thermodynamic parameters of transition (, , ) and in the appearance of a low temperature peak, that is practically irreversible against rescanning. All these indicate that the important defects in the molecule occur. The ESR measurements have shown that the above-mentioned thermal changes are connected with the occurrence of free radicals in solution under UV irradiation. They interact with proline (Pro) residues of the protein with the appearance of secondary free radicals, with following migration to glycine (Gly) residues. The emergence of the free radicals at the Pro and then at the Gly residues may cause the dramatic structural defect resulting from the UV irradiation, which significantly alters the network of hydrogen bonds in the triple helix of the collagen molecule. All this is connected with destabilization of the collagen molecule, because the defects in amino acid residues probably lead to cleavage of covalent bonds near the damaged sites maintaining the triple helical structure. The presence of ascorbic acid in collagen solution protects the collagen molecule from occurring of secondary free radicals.
- T. Miyata, T. Sohde, A. L. Rubin, and K. H. Stenzel, “Effects of ultraviolet irradiation on native and telopeptide-poor collagen,” Biochimica et Biophysica Acta, vol. 229, no. 3, pp. 672–680, 1971.
- T. Hyashi, S. Curran-Patel, and D. J. Prockop, “Thermal stability of the triple helix of type I procollagen and collagen. Precautions for minimizing ultraviolet damage to proteins during circular dichroism studies,” Biochemistry, vol. 18, no. 19, pp. 4182–4187, 1979.
- E. Fujimori, “Changes induced by ozone and ultraviolet light in type I collagen. Bovine Achilles tendon collagen versus rat tail tendon collagen,” European Journal of Biochemistry, vol. 152, no. 2, pp. 299–306, 1985.
- A. Kamińska and A. Sionkowska, “The effect of UV radiation on the thermal parameters of collagen degradation,” Polymer Degradation and Stability, vol. 51, no. 1, pp. 15–18, 1996.
- A. Sionkowska and A. Kamińska, “Thermal helix-coil transition in UV irradiated collagen from rat tail tendon,” International Journal of Biological Macromolecules, vol. 24, no. 4, pp. 337–340, 1999.
- C. A. Miles, A. Sionkowska, S. L. Hulin, T. J. Sims, N. C. Avery, and A. J. Bailey, “Identification of an intermediate state in the helix-coil degradation of collagen by ultraviolet light,” Journal of Biological Chemistry, vol. 275, no. 42, pp. 33014–33020, 2000.
- A. Sionkowska, “Flash photolysis and pulse radiolysis studies on collagen Type I in acetic acid solution,” Journal of Photochemistry and Photobiology B: Biology, vol. 84, no. 1, pp. 38–45, 2006.
- A. Sionkowska and T. Wess, “Mechanical properties of UV irradiated rat tail tendon (RTT) collagen,” International Journal of Biological Macromolecules, vol. 34, no. 1-2, pp. 9–12, 2004.
- K. Vizarova, D. Bakos, M. Rehakova, and V. Macho, “Modification of layered atelocollagen by ultraviolet irradiation and chemical cross-linking: structure stability and mechanical properties,” Biomaterials, vol. 15, no. 13, pp. 1082–1086, 1994.
- A. Sionkowska, “Modification of collagen films by ultraviolet irradiation,” Polymer Degradation and Stability, vol. 68, no. 2, pp. 147–151, 2000.
- M. Kitai, E. Sobol, A. Sviridov, and A. Omel'chenko, “Photochemical reactions in bone tissue induced by ultraviolet radiation of excimer laser,” Biofizika, vol. 41, pp. 1137–1144, 1996.
- Y. Tsuboi, N. Kimoto, M. Kabeshita, and A. Itaya, “Pulsed laser deposition of collagen and keratin,” Journal of Photochemistry and Photobiology A: Chemistry, vol. 145, no. 3, pp. 209–214, 2001.
- K. Tsunoda, D. Kumaki, T. Takahashi, H. Yajima, T. Ishii, and H. Itoh, “Characterization of materials ejected by excimer laser ablation of hydrated collagen gel,” Applied Surface Science, vol. 197-198, pp. 782–785, 2002.
- S. Lazare, V. Tokarev, A. Sionkowska, and M. Wiśniewski, “Surface foaming of collagen, chitosan and other biopolymer films by KrF excimer laser ablation in the photomechanical regime,” Applied Physics A: Materials Science and Processing, vol. 81, no. 3, pp. 465–470, 2005.
- A. Kamińska and A. Sionkowska, “Photochemical transformations in collagen in the presence of -carotene,” Journal of Photochemistry and Photobiology A: Chemistry, vol. 96, no. 1–3, pp. 123–127, 1996.
- A. Sionkowska and A. Kamińska, “Changes induced by ultraviolet light in fluorescence of collagen in the presence of -carotene,” Journal of Photochemistry and Photobiology A: Chemistry, vol. 120, no. 3, pp. 207–210, 1999.
- Y. Kato, K. Uchida, and S. Kawakishi, “Aggregation of collagen exposed to UVA in the presence of riboflavin: a plausible role of tyrosine modification,” Photochemistry and Photobiology, vol. 59, no. 3, pp. 343–349, 1994.
- A. Sionkowska, “Photochemical transformations in collagen in the presence of melanin,” Journal of Photochemistry and Photobiology A: Chemistry, vol. 124, no. 1-2, pp. 91–94, 1999.
- A. Sionkowska, “Influence of methylene blue on the photochemical stability of collagen,” Polymer Degradation and Stability, vol. 67, no. 1, pp. 79–83, 2000.
- M. J. Glimcher, C. J. Francois, L. Richards, and S. M. Krane, “The presence of organic phosphorus in collagens and gelatins,” Biochimica et Biophysica Acta, vol. 93, no. 3, pp. 585–602, 1964.
- N. Metreveli, L. Namicheishvili, K. Jariashvili, I. Jorjishvili, and G. Mrevlishvili, “The influence of ultra-violet irradiation on collagen,” Bulletin of the Georgian Academy of Sciences, vol. 164, no. 3, pp. 538–540, 2001.
- N. Metreveli, K. Jariashvili, L. Namicheishvili, and G. Mrevlishvili, “The influence of UV irradiation on collagen in the presence of vitamin C,” Bulletin of the Georgian Academy of Sciences, vol. 168, no. 3, pp. 546–548, 2003.
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