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International Journal of Photoenergy
Volume 2012, Article ID 192380, 8 pages
http://dx.doi.org/10.1155/2012/192380
Research Article

Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study

1Institute of Modern Optics, Nankai University, Tianjin 300071, China
2School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3R&D Department, Beijing GK Laser Technology Co., Ltd., No. 66 Xixiaokou Road, Beijing 100192, China

Received 13 July 2011; Accepted 10 October 2011

Academic Editor: Peter Robertson

Copyright © 2012 Ping Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorin f (CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubation time, which was followed by its binding to cell organelles. Subcellular distribution study revealed that CPD4 was primarily localized on the mitochondria and membranes, supporting that the mode of transmembrane transport was diffusion. A kinetics model describing CPD4 passing through the plasma membrane of MCF-7 cells was proposed based on Fick's first law of diffusion. The kinetics of cellular uptake of CPD4 was studied by three-dimensional LSCM. By fitting the experimental data using the above model, important cellular uptake and distribution parameters were obtained, which are of clinical significance in photodynamic therapy.