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International Journal of Photoenergy
Volume 2012 (2012), Article ID 275209, 5 pages
http://dx.doi.org/10.1155/2012/275209
Research Article

Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes

1Laboratory of Laser Sports Medicine, South China Normal University, Guangzhou 510631, China
2School of Life Science, South China Normal University, Guangzhou 510631, China
3The Key Laboratory of Ministry of Education of China in Laser Life Science, South China Normal University, Guangzhou 510631, China
4School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China
5Medical School, Jinan University, Guangzhou 510632, China

Received 30 January 2012; Revised 12 April 2012; Accepted 12 April 2012

Academic Editor: Rui Duan

Copyright © 2012 Gang-Yue Luo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2 for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2 for 5 min. Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer. It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness. The LHNL at 4.4 mW/cm2 for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2 (P<0.05). It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.