Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer

Figure 4

Comet assay measuring the DNA damage without using any enzyme, with using Endonuclease III and FPG protein for the five experimental groups of (a) A549, (b) MCF7, and (c) SNO cancer cells. In all three cell types the cells showed significant DNA damage after PDT treatment while cells that were pre-sensitized displayed less DNA damage compared to the PDT-treated cells. The sensitivity of the comet assay is enhanced significantly by using Endonuclease II and FPG proteins (***𝑃<0.001; **𝑃<0.05; *𝑃<0.01).

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(b)

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