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International Journal of Photoenergy
Volume 2013, Article ID 954914, 7 pages
Research Article

Bactericidal Activity of TiO2 on Cells of Pseudomonas aeruginosa ATCC 27853

1Facultad de Ingeniería, Universidad Autónoma de Querétaro, 76010 QRO, Mexico
2Facultad de Química, Materiales, Universidad Autónoma de Querétaro, 76010 QRO, Mexico

Received 25 May 2013; Revised 21 July 2013; Accepted 21 July 2013

Academic Editor: Guisheng Li

Copyright © 2013 J. L. Aguilar Salinas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The photocatalytic activity of semiconductors is increasingly being used to disinfect water, air, soils, and surfaces. Titanium dioxide (TiO2) is widely used as a photocatalyst in thin films, powder, and in mixtures with other semiconductors or metals. This work presents the antibacterial effects of TiO2 and light exposure (at 365 nm) on Pseudomonas aeruginosa ATCC 27853. TiO2 powder was prepared from a mixture of titanium isopropoxide, ethanol, and nitric acid using a green and short time sol-gel technique. The obtained gel annealed at 450°C was characterized by X-ray diffraction, Raman spectroscopy, ultraviolet-visible spectroscopy, diffuse reflectance, scanning electron microscopy, and transmission electron microscopy. The nanocomposite effectively catalyzed the inactivation of Pseudomonas aeruginosa. Following 90 minutes exposure to TiO2 and UV light, logarithm of cell density was reduced from 6 to 3. These results were confirmed by a factorial design incorporating two experimental replicates and two independent factors.