Multiorganelle Localization of Metallated Phthalocyanine Photosensitizer in Colorectal Cancer Cells (DLD-1 and CaCo-2) Enhances Efficacy of Photodynamic Therapy

Table 2

Cathepsin D and cytosolic cytochrome C were measured after 1 and 24 h incubation after PDT. Significant differences shown in the table represent comparison between PDT treated cells and their respective untreated control cells after 1 or 24 h incubation (^∗𝑃<0.05; ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001). Cathepsin D was significantly increased in DLD-1 (𝑃<0.01) and CaCo-2 (𝑃<0.05) after 24 h incubation in comparison to after 1 h incubation. In both cell lines, cytochrome C was significantly increased after 24 h incubation as compared to 1 h incubation (𝑃<0.01).

Assay	Time	Untreated		5 J/cm²		ZnPcSmix		PDT
		DLD-1	CaCo-2	DLD-1	CaCo-2	DLD-1	CaCo-2	DLD-1	CaCo-2
Cath-D	1 h	0.05^a±1.16^b	0.06±2.84	0.06±2.52	0.06±1.78	0.06^∗±9.76	0.07^∗±2.28	0.07^∗±3.82	0.08^∗∗±4.31
	24 h	0.06±2.39	0.07±1.31	0.06±3.03	0.06±9.38	0.07±5.12	0.07±2.02	0.10^∗∗∗±2.78	0.09^∗∗∗±3.89
Cyto-C	1 h	0.10±2.194	0.24±0.15	0.12±0.02	0.11±0.01	0.20^∗∗∗±0.03	0.11±0.02	2.41^∗∗∗±0.12	2.22^∗∗∗±0.18
	24 h	0.09±4.33	0.09±4.57	0.12±0.01	0.09±5.47	0.27±0.09	0.14±0.02	3.14^∗∗∗±0.22	3.05^∗∗∗±0.22

aMean; ^bstandard error of mean (SEM).