Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B
Figure 3
Molecular analyses of representative transgenic Miscanthus plants with PHYB. (a) Genomic PCR analysis of transgenic lines. The coding regions of the PHYB and BAR transgenes were amplified by PCR from genomic DNA. The actin gene (ACT) of M. sinensis plants was shown as a loading control of the genomic DNA. NT: nontransformed control plant; V: pCAMBIA3301 harboring PHYB gene that was used for transformation. (b) Southern blot analysis of transgenic lines. Genomic DNA (15 μg) from each transgenic plant was digested with either HindIII or EcoRI and then probed with the BAR gene. Numbers in lanes represent independent transgenic lines selected for analysis, and asterisks indicate the BAR bands. (c) Northern blot analysis of transgenic lines. Total RNA was isolated from the leaves of transgenic plants and the PHYB gene was used as a probe. Total RNA was shown as a loading control.
