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International Journal of Photoenergy
Volume 2014, Article ID 517510, 7 pages
Research Article

Scattered and Fluorescent Photon Track Reconstruction in a Biological Tissue

1A. M. Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilov Street 38, Moscow 119991, Russia
2Université de Lorraine, CRAN, UMR 7039, 2 avenue de la Forêt de Haye, 54516 Vandoeuvre-Lès-Nancy Cedex, France
3CNRS, CRAN, UMR 7039, 2 avenue de la Forêt de Haye, 54516 Vandoeuvre-Lès-Nancy Cedex, France

Received 6 November 2013; Revised 13 February 2014; Accepted 15 February 2014; Published 25 March 2014

Academic Editor: Alexandre Douplik

Copyright © 2014 Maria N. Kholodtsova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Appropriate analysis of biological tissue deep regions is important for tumor targeting. This paper is concentrated on photons’ paths analysis in such biotissue as brain, because optical probing depth of fluorescent and excitation radiation differs. A method for photon track reconstruction was developed. Images were captured focusing on the transparent wall close and parallel to the source fibres, placed in brain tissue phantoms. The images were processed to reconstruct the photons most probable paths between two fibres. Results were compared with Monte Carlo simulations and diffusion approximation of the radiative transfer equation. It was shown that the excitation radiation optical probing depth is twice more than for the fluorescent photons. The way of fluorescent radiation spreading was discussed. Because of fluorescent and excitation radiation spreads in different ways, and the effective anisotropy factor, , was proposed for fluorescent radiation. For the brain tissue phantoms it were found to be and for the irradiation wavelengths 532 nm and 632.8 nm, respectively. These calculations give more accurate information about the tumor location in biotissue. Reconstruction of photon paths allows fluorescent and excitation probing depths determination. The can be used as simplified parameter for calculations of fluorescence probing depth.