Effect and Mechanism of 808 nm Light Pretreatment of Hypoxic Primary Neurons

Figure 4

Effect of 25 mW/cm² 808 nm LED light pretreatment on COX activity. After cultured neuron cells being digested and collected, mitochondria isolation assay was added. 15 minutes later, cells were homogenized. After being centrifuged (600 g) for 10 minutes, supernatant was collected. After being centrifuged (11000 g) for 10 minutes, the precipitation was collected. Mitochondrial lysate was added to obtain the samples. Then COX was measured. Data are Means ± SEM. **(𝑃<0.01) and *(𝑃<0.05) mean compared with control group; ^##(𝑃<0.01) and ^#(𝑃<0.05) mean compared with 100 μM/L CoCl₂ group.