Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro

Table 3

Gene expression profile in normal (N) cells irradiated at 660 nm. Total RNA from nonirradiated N cells and irradiated N cells were characterised in triplicate. A fold difference >1 is considered as gene upregulation, while a fold difference <1 is considered as gene downregulation. Fold differences are indicated as upregulation (↑) or downregulation (↓). X denotes the nonsignificant genes in that particular cell model.

Gene symbol	Gene description	Gene ID	Fold difference	Up/downregulation	P value
ADAMTS1	ADAM metallopeptidase with thrombospondin type 1 motif, 1	9510	4.36	↑	0.003
ADAMTS13	ADAM metallopeptidase with thrombospondin type 1 motif, 13	11093	1.32	X	0.259
ADAMTS8	ADAM metallopeptidase with thrombospondin type 1 motif, 8	11095	2.77	↑	0.003
CD44	CD44 molecule (Indian blood group)	960	4.85	↑	0.012
CDH1	Cadherin 1, type 1, E-cadherin (epithelial)	999	0.58	↓	0.019
CLEC3B	C-type lectin domain family 3, member B	7123	1.90	X	0.078
CNTN1	Contactin 1	1272	1.14	X	0.547
COL11A1	Collagen, type XI, alpha 1	1301	1.05	X	0.896
COL12A1	Collagen, type XII, alpha 1	1303	2.21	↑	0.003
COL14A1	Collagen, type XIV, alpha 1	7373	0.56	X	0.116
COL15A1	Collagen, type XV, alpha 1	1306	1.33	X	0.198
COL16A1	Collagen, type XVI, alpha 1	1307	1.57	X	0.074
COL1A1	Collagen, type I, alpha 1	1277	1.88	↑	0.001
COL4A2	Collagen, type IV, alpha 2	1284	1.78	↑	0.022
COL5A1	Collagen, type V, alpha 1	1289	4.23	↑	0.001
COL6A1	Collagen, type VI, alpha 1	1291	2.42	↑	0.010
COL6A2	Collagen, type VI, alpha 2	1292	3.99	↑	0.000
COL7A1	Collagen, type VII, alpha 1	1294	1.13	X	0.658
COL8A1	Collagen, type VIII, alpha 1	1295	3.07	↑	0.000
CTGF	Connective tissue growth factor	1490	0.43	↓	0.002
CTNNA1	Catenin (cadherin-associated protein), alpha 1, 102 kDa	1495	0.73	↓	0.039
CTNNB1	Catenin (cadherin-associated protein), beta 1, 88 kDa	1499	1.27	X	0.153
CTNND1	Catenin (cadherin-associated protein), delta 1	1500	2.21	↑	0.001
CTNND2	Catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein)	1501	0.09	↓	0.002
ECM1	Extracellular matrix protein 1	1893	0.47	↓	0.000
FN1	Fibronectin 1	2335	2.29	↑	0.005
HAS1	Hyaluronan synthase 1	3036	1.20	X	0.182
ICAM1	Intercellular adhesion molecule 1	3383	0.72	X	0.156
ITGA1	Integrin, alpha 1	3672	1.88	↑	0.002
ITGA2	Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)	3673	0.99	X	0.650
ITGA3	Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)	3675	2.98	↑	0.000
ITGA4	Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)	3676	0.81	X	0.193
ITGA5	Integrin, alpha 5 (fibronectin receptor, alpha polypeptide)	3678	3.26	↑	0.000
ITGA6	Integrin, alpha 6	3655	1.21	X	0.085
ITGA7	Integrin, alpha 7	3679	0.92	X	0.780
ITGA8	Integrin, alpha 8	8516	1.06	X	0.805
ITGAL	Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)	3683	1.06	X	0.540
ITGAM	Integrin, alpha M (complement component 3 receptor 3 subunit)	3684	1.58	X	0.055
ITGAV	Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)	3685	1.17	X	0.331
ITGB1	Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)	3688	0.92	X	0.459
ITGB2	Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)	3689	0.96	X	0.792
ITGB3	Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)	3690	1.08	X	0.339
ITGB4	Integrin, beta 4	3691	1.25	X	0.127
ITGB5	Integrin, beta 5	3693	1.05	X	0.672
KAL1	Kallmann syndrome 1 sequence	3730	1.53	↑	0.030
LAMA1	Laminin, alpha 1	284217	0.52	↓	0.001
LAMA2	Laminin, alpha 2	3908	0.52	↓	0.008
LAMA3	Laminin, alpha 33909	3909	2.60	↑	0.023
LAMB1	Laminin, beta 1	3912	0.92	X	0.594
LAMB3	Laminin, beta 3	3914	0.55	X	0.069
LAMC1	Laminin, gamma 1 (formerly LAMB2)	3915	0.83	X	0.063
MMP1	Matrix metallopeptidase 1 (interstitial collagenase)	4312	0.41	↓	0.001
MMP10	Matrix metallopeptidase 10 (stromelysin 2)	4319	0.81	X	0.198
MMP11	Matrix metallopeptidase 11 (stromelysin 3)	4320	1.53	↑	0.015
MMP12	Matrix metallopeptidase 12 (macrophage elastase)	4321	0.48	↓	0.010
MMP13	Matrix metallopeptidase 13 (collagenase 3)	4322	0.42	↓	0.001
MMP14	Matrix metallopeptidase 14 (membrane-inserted)	4323	4.14	↑	0.000
MMP15	Matrix metallopeptidase 15 (membrane-inserted)	4324	1.96	↑	0.000
MMP16	Matrix metallopeptidase 16 (membrane-inserted)	4325	1.11	X	0.186
MMP2	Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase)	4313	3.34	↑	0.000
MMP3	Matrix metallopeptidase 3 (stromelysin 1, progelatinase)	4314	0.23	↓	0.007
MMP7	Matrix metallopeptidase 7 (matrilysin, uterine)	4316	0.62	↓	0.045
MMP8	Matrix metallopeptidase 8 (neutrophil collagenase)	4317	0.75	X	0.112
MMP9	Matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase)	4318	0.39	↓	0.013
NCAM1	Neural cell adhesion molecule 1	4684	1.63	↑	0.027
PECAM1	Platelet/endothelial cell adhesion molecule	5175	0.81	X	0.503
SELE	Selectin E	6401	0.93	X	0.568
SELL	Selectin L	6402	0.99	X	0.961
SELP	Selectin P (granule membrane protein 140 kDa, antigen CD62)	6403	1.35	X	0.469
SGCE	Sarcoglycan, epsilon	8910	0.36	↓	0.004
SPARC	Secreted protein, acidic, cysteine-rich (osteonectin)	6678	3.33	↑	0.000
SPG7	Spastic paraplegia 7 (pure and complicated autosomal recessive)	6687	5.12	↑	0.000
SPP1	Secreted phosphoprotein 1	6696	0.55	↓	0.001
TGFBI	Transforming growth factor, beta-induced, 68 kDa	7045	0.87	X	0.361
THBS1	Thrombospondin 1	7057	1.87	↑	0.006
THBS2	Thrombospondin 2	7058	2.65	↑	0.003
THBS3	Thrombospondin 3	7059	1.20	X	0.235
TIMP1	TIMP metallopeptidase inhibitor 1	7076	1.43	↑	0.035
TIMP2	TIMP metallopeptidase inhibitor 2	7077	1.17	↑	0.025
TIMP3	TIMP metallopeptidase inhibitor 3	7078	0.43	↓	0.008
TNC	Tenascin C	3371	0.49	↓	0.002
VCAM1	Vascular cell adhesion molecule 1	7412	0.38	↓	0.010
VCAN	Versican	1462	0.46	↓	0.008
VTN	Vitronectin	7448	1.12	X	0.585