Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro

Table 4

Gene expression profile in normal wounded (NW) cells irradiated at 660 nm. Total RNA from nonirradiated N cells and irradiated NW cells were characterised in triplicate. A fold difference >1 is considered as gene upregulation, while a fold difference <1 is considered as gene downregulation. Fold differences are indicated as upregulation (↑) or downregulation (↓). X denotes the nonsignificant genes in that particular cell model.

Gene symbol	Gene description	Gene ID	Fold difference	Up/downregulation	P value
ADAMTS1	ADAM metallopeptidase with thrombospondin type 1 motif, 1	9510	1.86	↑	0.045
ADAMTS13	ADAM metallopeptidase with thrombospondin type 1 motif, 13	11093	2.39	↑	0.003
ADAMTS8	ADAM metallopeptidase with thrombospondin type 1 motif, 8	11095	1.85	↑	0.001
CD44	CD44 molecule (Indian blood group)	960	1.25	X	0.356
CDH1	Cadherin 1, type 1, E-cadherin (epithelial)	999	1.23	X	0.205
CLEC3B	C-type lectin domain family 3, member B	7123	1.15	X	0.299
CNTN1	Contactin 1	1272	1.25	X	0.068
COL11A1	Collagen, type XI, alpha 1	1301	1.04	X	0.854
COL12A1	Collagen, type XII, alpha 1	1303	1.69	↑	0.004
COL14A1	Collagen, type XIV, alpha 1	7373	0.81	X	0.370
COL15A1	Collagen, type XV, alpha 1	1306	1.20	X	0.216
COL16A1	Collagen, type XVI, alpha 1	1307	1.05	X	0.749
COL1A1	Collagen, type I, alpha 1	1277	1.45	↑	0.006
COL4A2	Collagen, type IV, alpha 2	1284	1.44	X	0.069
COL5A1	Collagen, type V, alpha 1	1289	1.61	↑	0.005
COL6A1	Collagen, type VI, alpha 1	1291	1.27	X	0.170
COL6A2	Collagen, type VI, alpha 2	1292	1.36	X	0.105
COL7A1	Collagen, type VII, alpha 1	1294	1.42	↑	0.005
COL8A1	Collagen, type VIII, alpha 1	1295	1.36	↑	0.011
CTGF	Connective tissue growth factor	1490	0.95	X	0.284
CTNNA1	Catenin (cadherin-associated protein), alpha 1, 102 kDa	1495	1.03	X	0.720
CTNNB1	Catenin (cadherin-associated protein), beta 1, 88 kDa	1499	0.95	X	0.700
CTNND1	Catenin (cadherin-associated protein), delta 1	1500	1.36	X	0.105
CTNND2	Catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein)	1501	0.63	↓	0.019
ECM1	Extracellular matrix protein 1	1893	0.75	↓	0.017
FN1	Fibronectin 1	2335	1.63	↑	0.012
HAS1	Hyaluronan synthase 1	3036	2.00	↑	0.005
ICAM1	Intercellular adhesion molecule 1	3383	0.78	X	0.139
ITGA1	Integrin, alpha 1	3672	1.57	↑	0.005
ITGA2	Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)	3673	0.86	X	0.073
ITGA3	Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)	3675	1.33	X	0.121
ITGA4	Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)	3676	1.19	X	0.057
ITGA5	Integrin, alpha 5 (fibronectin receptor, alpha polypeptide)	3678	1.46	↑	0.021
ITGA6	Integrin, alpha 6	3655	1.55	↑	0.016
ITGA7	Integrin, alpha 7	3679	0.90	X	0.462
ITGA8	Integrin, alpha 8	8516	2.94	↑	0.012
ITGAL	Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) 3683	3683	2.04	↑	0.030
ITGAM	Integrin, alpha M (complement component 3 receptor 3 subunit) 3684	3684	1.59	↑	0.027
ITGAV	Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)	3685	1.43	↑	0.013
ITGB1	Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)	3688	1.05	X	0.652
ITGB2	Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)	3689	1.17	X	0.259
ITGB3	Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)	3690	1.53	↑	0.007
ITGB4	Integrin, beta 4	3691	1.42	X	0.062
ITGB5	Integrin, beta 5	3693	1.07	X	0.539
KAL1	Kallmann syndrome 1 sequence	3730	1.41	↑	0.006
LAMA1	Laminin, alpha 1	284217	0.81	X	0.096
LAMA2	Laminin, alpha 2	3908	1.01	X	0.921
LAMA3	Laminin, alpha 33909	3909	1.33	X	0.546
LAMB1	Laminin, beta 1	3912	1.18	X	0.113
LAMB3	Laminin, beta 3	3914	0.87	X	0.273
LAMC1	Laminin, gamma 1 (formerly LAMB2)	3915	0.94	X	0.419
MMP1	Matrix metallopeptidase 1 (interstitial collagenase)	4312	0.53	↓	0.011
MMP10	Matrix metallopeptidase 10 (stromelysin 2)	4319	1.06	X	0.456
MMP11	Matrix metallopeptidase 11 (stromelysin 3)	4320	1.60	↑	0.009
MMP12	Matrix metallopeptidase 12 (macrophage elastase)	4321	0.75	X	0.019
MMP13	Matrix metallopeptidase 13 (collagenase 3)	4322	0.78	X	0.075
MMP14	Matrix metallopeptidase 14 (membrane-inserted)	4323	0.97	X	0.909
MMP15	Matrix metallopeptidase 15 (membrane-inserted)	4324	2.66	↑	0.000
MMP16	Matrix metallopeptidase 16 (membrane-inserted)	4325	1.00	X	0.963
MMP2	Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase)	4313	1.52	↑	0.004
MMP3	Matrix metallopeptidase 3 (stromelysin 1, progelatinase)	4314	0.33	↓	0.005
MMP7	Matrix metallopeptidase 7 (matrilysin, uterine)	4316	1.15	X	0.499
MMP8	Matrix metallopeptidase 8 (neutrophil collagenase)	4317	1.53	↑	0.049
MMP9	Matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase)	4318	1.02	X	0.803
NCAM1	Neural cell adhesion molecule 1	4684	1.09	X	0.475
PECAM1	Platelet/endothelial cell adhesion molecule	5175	2.24	↑	0.009
SELE	Selectin E	6401	1.39	↑	0.041
SELL	Selectin L	6402	1.08	X	0.597
SELP	Selectin P (granule membrane protein 140 kDa, antigen CD62)	6403	1.47	↑	0.032
SGCE	Sarcoglycan, epsilon	8910	0.73	↓	0.034
SPARC	Secreted protein, acidic, cysteine-rich (osteonectin)	6678	1.59	↑	0.001
SPG7	Spastic paraplegia 7 (pure and complicated autosomal recessive)	6687	1.28	↑	0.042
SPP1	Secreted phosphoprotein 1	6696	0.84	X	0.118
TGFBI	Transforming growth factor, beta-induced, 68 kDa	7045	1.10	X	0.317
THBS1	Thrombospondin 1	7057	1.59	↑	0.008
THBS2	Thrombospondin 2	7058	2.04	↑	0.004
THBS3	Thrombospondin 3	7059	0.89	X	0.349
TIMP1	TIMP metallopeptidase inhibitor 1	7076	1.31	X	0.073
TIMP2	TIMP metallopeptidase inhibitor 2	7077	1.07	X	0.244
TIMP3	TIMP metallopeptidase inhibitor 3	7078	0.89	X	0.340
TNC	Tenascin C	3371	0.73	↓	0.005
VCAM1	Vascular cell adhesion molecule 1	7412	0.61	X	0.065
VCAN	Versican	1462	0.87	X	0.252
VTN	Vitronectin	7448	0.62	↓	0.024