Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro

Table 5

Gene expression profile in diabetic wounded (DW) cells irradiated at 660 nm. Total RNA from nonirradiated DW cells and irradiated DW cells were characterised in triplicate. A fold difference >1 is considered as gene upregulation, while a fold difference <1 is considered as gene downregulation. Fold differences are indicated as upregulation (↑) or downregulation (↓). X denotes the nonsignificant genes in that particular cell model.

Gene symbol	Gene description	Gene ID	Fold difference	Up/downregulation	P value
ADAMTS1	ADAM metallopeptidase with thrombospondin type 1 motif, 1	9510	0.50	↓	0.003
ADAMTS13	ADAM metallopeptidase with thrombospondin type 1 motif, 13	11093	0.91	X	0.589
ADAMTS8	ADAM metallopeptidase with thrombospondin type 1 motif, 8	11095	2.75	↑	0.005
CD44	CD44 molecule (Indian blood group)	960	0.53	↓	0.049
CDH1	Cadherin 1, type 1, E-cadherin (epithelial)	999	2.12	↑	0.051
CLEC3B	C-type lectin domain family 3, member B	7123	1.14	X	0.749
CNTN1	Contactin 1	1272	2.19	↑	0.041
COL11A1	Collagen, type XI, alpha 1	1301	3.68	↑	0.002
COL12A1	Collagen, type XII, alpha 1	1303	0.37	X	0.003
COL14A1	Collagen, type XIV, alpha 1	7373	1.24	↑	0.023
COL15A1	Collagen, type XV, alpha 1	1306	0.67	X	0.082
COL16A1	Collagen, type XVI, alpha 1	1307	0.61	↓	0.001
COL1A1	Collagen, type I, alpha 1	1277	0.87	X	0.498
COL4A2	Collagen, type IV, alpha 2	1284	0.99	X	0.966
COL5A1	Collagen, type V, alpha 1	1289	0.56	↓	0.036
COL6A1	Collagen, type VI, alpha 1	1291	0.64	↓	0.015
COL6A2	Collagen, type VI, alpha 2	1292	0.58	↓	0.014
COL7A1	Collagen, type VII, alpha 1	1294	0.36	↓	0.017
COL8A1	Collagen, type VIII, alpha 1	1295	0.71	X	0.271
CTGF	Connective tissue growth factor	1490	1.10	X	0.521
CTNNA1	Catenin (cadherin-associated protein), alpha 1, 102 kDa	1495	0.83	X	0.340
CTNNB1	Catenin (cadherin-associated protein), beta 1, 88 kDa	1499	0.64	X	0.084
CTNND1	Catenin (cadherin-associated protein), delta 1	1500	0.36	↓	0.005
CTNND2	Catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein)	1501	2.72	↑	0.052
ECM1	Extracellular matrix protein 1	1893	0.74	X	0.076
FN1	Fibronectin 1	2335	0.58	↓	0.008
HAS1	Hyaluronan synthase 1	3036	0.87	X	0.544
ICAM1	Intercellular adhesion molecule 1	3383	0.97	X	0.843
ITGA1	Integrin, alpha 1	3672	0.97	X	0.721
ITGA2	Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)	3673	0.52	↓	0.015
ITGA3	Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)	3675	0.50	↓	0.010
ITGA4	Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)	3676	0.86	X	0.459
ITGA5	Integrin, alpha 5 (fibronectin receptor, alpha polypeptide)	3678	0.54	↓	0.004
ITGA6	Integrin, alpha 6	3655	0.76	↓	0.012
ITGA7	Integrin, alpha 7	3679	1.11	X	0.513
ITGA8	Integrin, alpha 8	8516	2.75	↑	0.051
ITGAL	Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) 3683	3683	1.89	↑	0.003
ITGAM	Integrin, alpha M (complement component 3 receptor 3 subunit)	3684	1.77	X	0.065
ITGAV	Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)	3685	0.50	↓	0.002
ITGB1	Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)	3688	0.53	↓	0.005
ITGB2	Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)	3689	1.58	X	0.083
ITGB3	Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)	3690	0.58	↓	0.006
ITGB4	Integrin, beta 4	3691	1.87	↑	0.041
ITGB5	Integrin, beta 5	3693	0.97	X	0.879
KAL1	Kallmann syndrome 1 sequence	3730	0.41	↓	0.001
LAMA1	Laminin, alpha 1	284217	0.60	↓	0.014
LAMA2	Laminin, alpha 2	3908	1.17	X	0.396
LAMA3	Laminin, alpha 3	3909	2.25	↑	0.031
LAMB1	Laminin, beta 1	3912	0.95	X	0.609
LAMB3	Laminin, beta 3	3914	0.61	↓	0.010
LAMC1	Laminin, gamma 1 (formerly LAMB2)	3915	0.54	↓	0.008
MMP1	Matrix metallopeptidase 1 (interstitial collagenase)	4312	0.38	↓	0.001
MMP10	Matrix metallopeptidase 10 (stromelysin 2)	4319	1.28	X	0.271
MMP11	Matrix metallopeptidase 11 (stromelysin 3)	4320	1.71	↑	0.025
MMP12	Matrix metallopeptidase 12 (macrophage elastase)	4321	0.52	↓	0.005
MMP13	Matrix metallopeptidase 13 (collagenase 3)	4322	1.89	↑	0.034
MMP14	Matrix metallopeptidase 14 (membrane-inserted)	4323	0.44	↓	0.016
MMP15	Matrix metallopeptidase 15 (membrane-inserted)	4324	1.24	X	0.157
MMP16	Matrix metallopeptidase 16 (membrane-inserted)	4325	0.44	↓	0.003
MMP2	Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase)	4313	0.54	↓	0.002
MMP3	Matrix metallopeptidase 3 (stromelysin 1, progelatinase)	4314	2.42	↑	0.009
MMP7	Matrix metallopeptidase 7 (matrilysin, uterine)	4316	1.86	↑	0.024
MMP8	Matrix metallopeptidase 8 (neutrophil collagenase)	4317	0.63	↓	0.029
MMP9	Matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase)	4318	1.47	↑	0.009
NCAM1	Neural cell adhesion molecule 1	4684	0.97	X	0.811
PECAM1	Platelet/endothelial cell adhesion molecule	5175	3.66	X	0.213
SELE	Selectin E	6401	1.50	X	0.078
SELL	Selectin L	6402	2.36	↑	0.013
SELP	Selectin P (granule membrane protein 140 kDa, antigen CD62)	6403	1.90	X	0.249
SGCE	Sarcoglycan, epsilon	8910	0.93	X	0.657
SPARC	Secreted protein, acidic, cysteine-rich (osteonectin)	6678	0.66	↓	0.054
SPG7	Spastic paraplegia 7 (pure and complicated autosomal recessive)	6687	0.48	↓	0.005
SPP1	Secreted phosphoprotein 1	6696	0.58	↓	0.009
TGFBI	Transforming growth factor, beta-induced, 68 kDa	7045	0.79	X	0.099
THBS1	Thrombospondin 1	7057	0.35	↓	0.001
THBS2	Thrombospondin 2	7058	0.81	X	0.088
THBS3	Thrombospondin 3	7059	1.03	X	0.794
TIMP1	TIMP metallopeptidase inhibitor 1	7076	1.47	↑	0.046
TIMP2	TIMP metallopeptidase inhibitor 2	7077	0.93	X	0.788
TIMP3	TIMP metallopeptidase inhibitor 3	7078	1.05	X	0.825
TNC	Tenascin C	3371	1.18	X	0.492
VCAM1	Vascular cell adhesion molecule 1	7412	1.79	↑	0.002
VCAN	Versican	1462	0.96	X	0.775
VTN	Vitronectin	7448	1.20	X	0.240