Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro

Table 6

Summary of the various genes significantly expressed and their pathways in normal (N), normal wounded (NW), and diabetic wounded (DW) cells. Fold differences are indicated as upregulation (↑) or downregulation (↓). X denotes the nonsignificant genes in that particular cell model.

Gene symbol	Gene description	Gene pathway	N	NW	DW
ADAMTS1	ADAM metallopeptidase with thrombospondin type 1 motif, 1	ECM protease	↑	X	X
ADAMTS13	ADAM metallopeptidase with thrombospondin type 1 motif, 13	ECM protease, cell-matrix adhesion	X	↑	X
ADAMTS8	ADAM metallopeptidase with thrombospondin type 1 motif, 8	ECM protease	↑	↑	↑
CD44	CD44 molecule (Indian blood group)	Transmembrane, cell-cell adhesion	↑	X	↓
CDH1	Cadherin 1, type 1, E-cadherin (epithelial)	Transmembrane, cell-cell adhesion	↓	X	↓
CLEC3B	C-type lectin domain family 3, member B	Other adhesion and ECM molecules	↑	X	X
CNTN1	Contactin 1	Other adhesion molecules	X	X	↑
COL11A1	Collagen, type XI, alpha 1	Cell-cell adhesion, collagen, and ECM structure constituent	X	X	↑
COL12A1	Collagen, type XII, alpha 1	Other adhesion molecules, collagen, and ECM structure constituent	↑	↑	↓
COL14A1	Collagen, type XIV, alpha 1	Cell-cell adhesion, collagen, and ECM structure constituent	X	X	↑
COL16A1	Collagen, type XVI, alpha 1	Other adhesion molecules, collagen, and ECM structure constituent	X	X	↓
COL1A1	Collagen, type I, alpha 1	Collagen and ECM structure constituent	↑	↑	X
COL4A2	Collagen, type IV, alpha 2	Basement membrane constituent and ECM structure constituent	↑	X	X
COL5A1	Collagen, type V, alpha 1	Other adhesion molecules, collagen, and ECM structure constituent	↑	↑	↓
COL6A1	Collagen, type VI, alpha 1	Other adhesion molecules, collagen, and ECM structure constituent	↑	X	↓
COL6A2	Collagen, type VI, alpha 2	Cell-cell adhesion, collagen, and ECM structure constituent	↑	X	↓
COL7A1	Collagen, type VII, alpha 1	Other adhesion molecules, basement membrane constituent, collagen, ECM structure constituent, and ECM protease inhibitor	X	X	↓
COL8A1	Collagen, type VIII, alpha 1	Other adhesion molecules, collagen, and ECM structure constituent	↑	↑	X
CTGF	Connective tissue growth factor	Other adhesion and ECM molecules	↓	X	X
CTNNA1	Catenin (cadherin-associated protein), alpha 1, 102 kDa	Other adhesion molecules	↓	X	X
CTNND1	Catenin (cadherin-associated protein), delta 1	Cell-cell adhesion	↑	X	↓
CTNND2	Catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein)	Other adhesion molecules	↓	↓	↑
ECM1	Extracellular matrix protein 1	Other ECM molecules	↓	↓	X
FN1	Fibronectin 1	Other adhesion molecules, collagen, and ECM structure constituent	↑	↑	↓
HAS1	Hyaluronan synthase 1	Transmembrane and other ECM molecules	X	↑	X
ITGA1	Integrin, alpha 1	Transmembrane	↑	↑	X
ITGA2	Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)	Transmembrane	X	X	↓
ITGA3	Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)	Transmembrane	↑	X	↓
ITGA5	Integrin, alpha 5 (fibronectin receptor, alpha polypeptide)	Transmembrane	↑	↑	↓
ITGA6	Integrin, alpha 6	Transmembrane	X	↑	↓
ITGA8	Integrin, alpha 8	Transmembrane and cell-cell adhesion	X	↑	↑
ITGAL	Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)	Transmembrane	X	↑	↑
ITGAM	Integrin, alpha M (complement component 3 receptor 3 subunit)	Transmembrane	X	↑	X
ITGAV	Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)	Transmembrane	X	↑	↓
ITGB1	Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 including MDF2, MSK12)	Transmembrane	X	X	↓
ITGB3	Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)	Transmembrane	X	↑	↓
ITGB4	Integrin, beta 4	Transmembrane	X	X	↑
KAL1	Kallmann syndrome 1 sequence	Other adhesion molecules, collagen, ECM structure constituent, and ECM protease inhibitor	↑	↑	↓
LAMA1	Laminin, alpha 1	Other adhesion molecules and basement membrane constituent	↓	X	X
LAMA2	Laminin, alpha 2	Other adhesion molecules and basement membrane constituent	↓	X	X
LAMA3	Laminin, alpha 33909	Other adhesion molecules and basement membrane constituent	↑	X	↑
LAMB3	Laminin, beta 3	Other adhesion molecules and basement membrane constituent	X	X	↓
LAMC1	Laminin, gamma 1 (formerly LAMB2)	Other adhesion molecules and basement membrane constituent	X	X	↓
MMP1	Matrix metallopeptidase 1 (interstitial collagenase)	ECM protease	↓	↓	↓
MMP11	Matrix metallopeptidase 11 (stromelysin 3)	ECM protease	↑	↑	↑
MMP12	Matrix metallopeptidase 12 (macrophage elastase)	ECM protease	↓	↓	↓
MMP13	Matrix metallopeptidase 13 (collagenase 3)	ECM protease	↓	X	↑
MMP14	Matrix metallopeptidase 14 (membrane-inserted)	Transmembrane and ECM protease	↑	X	↓
MMP15	Matrix metallopeptidase 15 (membrane-inserted)	Transmembrane and ECM protease	↑	↑	X
MMP16	Matrix metallopeptidase 16 (membrane-inserted)	Transmembrane and ECM protease	X	X	↓
MMP2	Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, and 72 kDa type IV collagenase)	ECM protease	↑	↑	↓
MMP3	Matrix metallopeptidase 3 (stromelysin 1 and progelatinase)	ECM protease	↓	↓	↑
MMP7	Matrix metallopeptidase 7 (matrilysin and uterine)	ECM protease	↓	X	↑
MMP8	Matrix metallopeptidase 8 (neutrophil collagenase)	ECM protease	X	↑	↓
MMP9	Matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, and 92 kDa type IV collagenase)	ECM protease	↓	X	↑
NCAM1	Neural cell adhesion molecule 1	Transmembrane	↑	X	X
PECAM1	Platelet/endothelial cell adhesion molecule	Transmembrane	X	↑	X
SELE	Selectin E	Transmembrane	X	↑	X
SELL	Selectin L	Transmembrane	X	X	↑
SELP	Selectin P (granule membrane protein 140 kDa and antigen CD62)	Transmembrane	X	↑	X
SGCE	Sarcoglycan, epsilon	Transmembrane	↓	↓	X
SPARC	Secreted protein, acidic, and cysteine-rich (osteonectin)	Basement membrane constituent	↑	↑	X
SPG7	Spastic paraplegia 7 (pure and complicated autosomal recessive)	Transmembrane and ECM protease	↑	↑	↓
SPP1	Secreted phosphoprotein 1	Cell-matrix adhesion and other ECM molecules	↓	X	↓
THBS1	Thrombospondin 1	Other adhesion molecules and ECM protease inhibitor	↑	↑	↓
THBS2	Thrombospondin 2	Other adhesion and ECM molecules	↑	↑	X
TIMP1	TIMP metallopeptidase inhibitor 1	ECM protease + inhibitor	↑	X	↑
TIMP2	TIMP metallopeptidase inhibitor 2	ECM protease inhibitor	↑	X	X
TIMP3	TIMP metallopeptidase inhibitor 3	ECM protease inhibitor	↓	X	X
TNC	Tenascin C	Other adhesion molecules	↓	↓	X
VCAM1	Vascular cell adhesion molecule 1	Transmembrane and cell-cell adhesion	↓	X	↑
VCAN	Versican	Other adhesion and ECM molecules	↓	X	X
VTN	Vitronectin	Other adhesion and ECM molecules	X	↓	X