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International Journal of Photoenergy
Volume 2014, Article ID 898752, 7 pages
http://dx.doi.org/10.1155/2014/898752
Research Article

Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells

1College of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
2CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
3Key Laboratory of System Biology, Chinese Academy of Sciences, Shanghai 201210, China
4Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

Received 9 January 2014; Accepted 15 February 2014; Published 6 April 2014

Academic Editor: Timon Cheng-Yi Liu

Copyright © 2014 Fu-qing Zhong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objective. ATP-sensitive potassium (KATP) channel couples cell metabolism to excitability. To explore role of KATP channels in cellular photobiomodulation, we designed experiment to study effect of low intensity 808 nm laser irradiation on the activity of membrane KATP channel. Study Design/Materials and Methods. Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells. The patch-clamp and data acquisition systems were used to record KATP channel current before and after irradiation. A laser beam of Ga-As 808 nm at 5 mW/cm2 was used in experiments. A one-way ANOVA test followed by a post hoc Student-Newman-Keuls test was used to assess the statistical differences between data groups. Results. Obvious openings of KATP channels of Kir6.2-transfected HEK-293 cells and excised patches were recorded during and after low intensity 808 nm laser irradiation. Compared with the channels that did not undergo irradiation, open probability, current amplitude, and dwell time of KATP channels after irradiation improved. Conclusions. Low intensity 808 nm laser irradiation may activate membrane KATP channels of Kir6.2-transfected HEK-293 cells and in excised patches.