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International Journal of Photoenergy
Volume 2014, Article ID 971491, 5 pages
Research Article

Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation

1College of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
2Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
3Key Laboratory of System Biology, Chinese Academy of Sciences, Shanghai 201210, China
4Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

Received 26 December 2013; Accepted 18 February 2014; Published 1 April 2014

Academic Editor: Timon Cheng-Yi Liu

Copyright © 2014 Shuang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system. Background Data. Treatment with light in the spectrum from red to near-infrared region has beneficial effect on tissue injury and 670 nm LED is currently under clinical investigation for retinoprotective therapy. Methods. Studies were conducted in the cultured cells under hypoxia treated by cobalt chloride (CoCl2). After irradiation by 670 nm LED with different power densities, cell viability, cytochrome C oxidase activity, and ATP concentration were measured. Results. The irradiation of 670 nm LED significantly improved cell viability, cytochrome C oxidase activity, and ATP concentration in the hypoxia RF/6A cells. Conclusion. 670 nm LED irradiation could recover the hypoxia damage caused by CoCl2. Photobiomodulation of 670 nm LED plays a potential role for the treatment of diabetic retinopathy.