Aggregation Behavior of Some Asymmetric Porphyrins versus Basic Biological Tests Response
Fractal analysis of free bases porphyrins was computed on atomic force microscopy (AFM) micrographs using two different methods: the correlation function method and the variable length scale method. The correlation function method provides fractal dimension only for short scale range; results indicate that only few images have fractal properties for short ranges; for the rest of them, no fractal dimension was found using the correlation function method. The variable length scale method occur information for long range scaling. All samples have fractal properties at higher scaling range. For three samples the correlation function method leads to the same fractal dimension as the variable length scale method and scaling ranges for both methods overlap. Results show the necessity to use both methods to describe the fractal properties of AB3 meso-porphyrins that may be used to predict their relative cell localization. In order to emphasize the influence of fractal and textural properties the results regarding their self-similarity and texture/morphology were further compared with their behavior in biological assessment, that is, functionality of some Jurkat cell lines.
Porphyrins are more and more involved in “long-chain” studies, with multiple different approaches of the subject, mainly in diagnosis and therapy of cancer, the emerging concept of theranostics, targeting the third generation of photosensitizers. The use of the porphyrin derivatives in biomedical field is a consequence of their ability to generate reactive species . The application domains are continuously expanding [2, 3] their use as sensitizers in photodynamic therapy of cancer being today the object of many interdisciplinary studies . Although a large number of meso-substituted porphyrins were synthesized since the approval of the first photosensitizer for clinical photodynamic therapy , there are still improvements to be made in their synthesis  and there is still no clear correlation between the mesosubstituents of porphyrins and their selective accumulation and phototoxicity in tumor cells. Recently it has been established that subcellular localization plays a major role in photodynamic efficacy .
We consider the balance between symmetry and asymmetry important, regarding the influence of peripheral substituents especially in tissue uptake. This view is supported by the fact that certain changes in the functional groups on a tetrapyrrolic macrocycle, including those attached in mesopositions, influence in significant way the reactivity and mechanism of the porphyrins involved in environmental reactions. Due to the extended electronic system, the porphyrinic behavior is marked by strong tendencies to form large aggregates . Most used methods to evaluate the capacity of self-association of this type of molecules are set around spectral analysis as liquid samples . Several parameters, as pH and solvent polarity, play a major role in aggregation capacity in the liquid state of the sample. By using AFM technique, these influential parameters are excluded. The binomial frame formed by AFM studies and porphyrin structures seem to be lately point of interest, most of them focusing on symmetric structures, free bases , from simple compounds  to advanced materials [12, 13]. From the behavior of porphyrins bind with peptides  to potential sensing devices , aspects as supramolecular chirality attract the attention via AFM studies. The variation of peripheral substituents of porphyrins has direct consequences on the formation of porphyrin aggregates, creating high supramolecular chirality targeting supramolecular networks that emulate the biological systems [16, 17]. One of the most detectable features of the porphyrins is the capacity to aggregate, through several types of interactions, where most of them are governed by the π-π interactions or hydrogen bonding. This characteristic is desirable for applications in technology, especially in nanotechnology . H-aggregates (face-to-face arrangement) and J-aggregates (side-by-side) are the well-known assemblies as shown in Figure 1, with structures optimized by HyperChem . Instead, for the biological applications as it theranostics is, the capacity to conglomerate is an obvious constraint of the membrane cell penetration process. AFM coupled with some cell lines tests proved to be a useful tool to evaluate the capacity of certain porphyrin to interact with the cell membrane. In this experiment the correlation is made between the scales of magnitude and is not related with the kind of aggregation.
“Scaling” is one of the modern concepts used to characterize the morphology of various complex surfaces, which can be smooth when the “observer” (i.e., the tip in scanning probe microscopy) is far enough and rough when it is close enough. Scaling relations characterize self-similar or self-affine objects and lead to direct computation of an important parameter: fractal dimension . Not all rough surfaces are fractals, but if a surface can be described in terms of fractal geometry, this means that it is self-affine in a statistical way and can be characterized by its fractal dimension. Fractal dimension gives information about the surface roughness, a higher fractal dimension meaning that the surface is very rough, but tells us something about surface geometry; that is, the surface is self-affine. This is a very important thing that when something seems to be disordered and hard to be described, self-affinity and fractal dimension give quantitative information about the surface and describe it. Fractal dimension can be measured in different ways: the direct method implies analysis of topographic images obtained from scanning tunneling microscopy [21–24], scanning probe microscopy , or atomic force microscopy [26–28], but there are a lot of other indirect methods: light scattering, neutron scattering, adsorption, and so on.
The aim of this paper is to analyze AFM topographic images of asymmetric porphyrins (AB3 meso-porphyrinic type structures) in order to analyze their fractal behavior and textural properties and to identify the connection with some results of biological tests. Two mathematical methods were used to compute fractal dimensions: first, the correlation function method [29–31] and, second, the variable length scale method . Our work emphasizes the interdependence between molecular architecture, complex structure analysis, and biological studies, especially AFM technique and porphyrin-cell interaction, in the quest for photosensitizers with improved properties. The order found in their aggregation tendencies is projected in the behavior observed in the cell interaction revealed by cytotoxicity tests.
2. Experimental Section
2.1. Materials and Methods
Several asymmetrically substituted porphyrins (Figure 2), 5-(3,4-methylenedyoxy)-phenyl-10,15,20-tris-phenyl-21,23H-porphine (TMDPP), 5-(3-hydroxy-4-methoxy)-phenyl-10,15,20-tris-phenyl-21,23H-porphine (TMHAPP), 5-(3-hydroxy-4-methoxy)-phenyl-10,15,20-tris-(3,4-methylenedyoxy)-21,23H-porphine (MHTPP), and 5-phenyl-10,15,20-tris-(3,4-methylenedyoxy)-phenyl-21,23H-porphine (TRMDOPP), and symmetrical compounds, 5,10,15,20-meso-tetrakis-(3,4-methylenedyoxy)-phenyl-21,23H porphine (TMDOPP) and 5,10,15,20-meso-tetrakis-(3-hydroxy-4-methoxy)-phenyl-21,23H-porphine (THAPP), were involved in the present studies.
For the series of AB3 asymmetrical mesoporphyrinic structures, details about synthetic technique are reported elsewhere [33–37]. The substituents attached to the porphyrinic tetrapyrrolic core were chosen so as to balance solubility, aggregation/textural tendencies, high singlet oxygen quantum yield, and cell interaction; two levels of asymmetry occur in the same individual structure: in the macrocyclic core and of the external substituents, complementary to several well-known “classical” macrocyclic porphyrin-type compounds.
(a) Characterization Methods. Atomic force microscopy (AFM) measurements were carried in true noncontact mode recommended for nondestructive sample scan with a XE-100 apparatus from Park Systems equipped with flexure-guided, cross talk eliminated scanners, using ultrasharp tips (<8 nm tip radius; NCHR type from NanosensorsTM) of 125 μm length, 30 μm width, and 42 N/m spring constant/~330 kHz resonance frequency. Different imaging scales were used from 8 × 8 μm2 to 1 × 1 μm2. In order to prepare the specimens for AFM investigations, a small quantity of powder was ultrasonically dispersed in ultrapure water (Millie-Q, >18 MΩcm), thus using the same concentration of the compounds as used in the biological investigations; despite the differences between the nature of solvents used for microscopy and cytotoxicity tests, this part of study chases only the tendencies of aggregation. A drop from this suspension was deposited on atomically flat Highly Oriented Pyrolytic Graphite (HOPG) and dried at room temperature. HOPG was used as to avoid any influence of the substrate on roughness and texture of the investigated samples. The Phase Contrast AFM images were processed by Scanning Probe Image Processor software (SPIPTM v. 18.104.22.168) (SPIP manual available at http://www.imagemet.com/).
(b) Correlation Function Method. A fractal is an object with an observed volume that depends on the resolution (length scale) and follows a power law behavior with a nontrivial exponent over several orders of magnitude. The most important property of fractals is self-similarity, which is the property of a part to look like the whole. Isotropic fractals are self-similar: they are invariant under isotropic scale transformation. When the object scales differently in different space directions, we call it a self-affine fractal. From this point of view, rough surfaces are usually self-affine structures . In our work we shall use to compute fractal dimensions two methods: the height correlation function method () and the variable length scale method () . Different parameters can be used to characterize the surface roughness. One of these parameters that describe self-affine surfaces is the roughness exponent . In addition to the roughness exponent , it is possible to associate a fractal dimension with a self-affine function. The fractal dimension of a self-affine surface can be computed from the height correlation function [29–31]:where the symbol denotes an average over and is defined asthe surface being described by the function which gives the maximum height of the interface at a position given by . Thus, the height correlation function obeys the following scaling relation :where, for a surface embedded in a 3-dimensional Euclidean space,with being the fractal dimension.
The scaling range in which (3) is obeyed is called the “cut-off” limits and it indicates the range of self-affinity, in other words, the range where there are correlations between the surface points. Correlation function method is suitable for small scaling range because it requires enough points for average in (1), meaning that points computed for low scaling range have low errors and points computed for high scaling range have high errors.
(c) Variable Length Scale Method. The model was proposed by Chauvy et al.  and consists of several steps: (i) defining an interval of length (or a box of size ); (ii) performing a linear (or planar) least square fit on the data within the interval and calculating the roughness; (iii) moving the interval (box) along the profile (surface) and repeating step (ii); (iv) computing Rms deviation for multiple intervals; and (v) repeating steps (ii)–(iv) for increasing lengths (box sizes). The smallest size for an interval corresponds to 10 data points ( points for 3-dimensional embedded objects) and its maximum size is the total length of the profile (size of the surface). Rms deviation , averaged over , the number of intervals of length , is defined bywhere is the th height variation from the best fit line within the interval and is the number of points in the interval .
The log-log plot of versus gives the Hurst or roughening exponent , and the fractal dimension can be calculated aswhere is the topological dimension of the embedding Euclidean space ( for profiles and for surfaces). Variable length scale method is suitable for higher scaling range compared to correlation function method because the necessity to have enough points in an interval to compute Rms deviation , averaged over , means that must be high enough for a good statistic. Both methods, correlation function method () and variable length scale () method, will be used to compute fractal dimension of topographic AFM images.
2.2. Preparation Method of Biological Samples
Preliminary in vitro cytotoxicity studies were performed on the Jurkat cell line, human T cell lymphoblast-like cell line. 10 mM stock solutions of the test porphyrinic compounds were prepared in DMSO by sonication at 22000 Hz for 30 seconds. For cellular tests stock solutions were further diluted in RPMI 1640 culture medium in the concentration range 1.25–40 μM. Solutions were handled in sterile conditions. Cell line was cultivated at 37°C, in 5% CO2 humid atmosphere, in RPMI 1640 medium supplemented with 100 U/mL penicillin, 0.1 mg/mL streptomycin, 0.25 μg/mL amphotericin, 2 mM glutamine, and 10% fetal bovine serum. For cellular viability and proliferation assays, Jurkat cells/mL was incubated for 3 h with various concentrations of compounds. The cellular control consisted in unloaded/untreated Jurkat cells. Cellular viability was assessed by measuring the bioreduction of a tetrazolium salt (MTS) to a formazan product, using CellTiter 96 AQeous One Solution Cell Proliferation Assay kit (Promega). The reaction, performed by dehydrogenase enzymes, takes place in metabolically active cells  and, therefore, MTS reduction is proportional to the number of viable cells.
Membrane integrity was measured by means of the lactate dehydrogenase (LDH) release assay , using CytoTox 96 Non-Radioactive Cytotoxicity Test (Promega). Briefly, cell culture supernatants were collected for the LDH release assay, whilst the rest of the cell suspension was used for the MTS reduction test. Optical densities (OD) were measured for triplicate samples on a Jasco V630 spectrophotometer, in a single beam mode, at 490 nm without any reference (for LDH release) and at 490 nm with reference at 640 nm (for MTS reduction). The mean value of triplicates was calculated and results were further expressed as percent effect relative to control.
3. Results and Discussion
Typical AFM images for the investigated porphyrins are presented in Figure 3, at the scale of μm2 for samples (1)–(6), in which the first column represents the 2D topographic images presented in Enhanced Color view mode and the second column the images recorded in Phase Contrast mode, and below them are the characteristic surface profiles (line scans). It was used the Enhanced Color view mode for the topographic 2D AFM images in order to increase the morphological details of the samples. This is because Enhanced Color uses the change of a pixel relative to its neighbors instead of its absolute value. Phase Contrast working mode was also registered in AFM experiments as to check for possible chemical inhomogeneities of the investigated compounds.
Some topographical characteristics that were consistently observed by AFM are discussed further. (1) TMHAPP is characterized by grains with height up to 10 nm and typical diameters in the range of 60–100 nm. (2) THAPP shows large grains (agglomerated particles) with diameters from 150 to 400 nm and height of around 20 nm (up to 30–40 of the larger ones). Between them, some small surface particles are visible (better in Amplitude or Phase Contrast images), with diameters in the range of 30–40 nm. There is already a visible tendency towards “self-assembling” for sample. (3) MHTPP is in terms of creating large “parcels” with mean height of around 16 nm and length in the microns range (aspect ratio of up to 1/100 – height/length). (4) TMDOPP exhibit the highest tendency towards agglomeration as could be seen in the corresponding 2D AFM image (topography), 4th row in Figure 3, which could be due to the presence of trace amounts of impurities. (5) TMDPP has also a strong tendency to form large “2D” islands of materials (the height of the largest island in the 5th row being 5 nm) instead of stacks as visible for sample (4), TMDOPP. (6) For sample TRMDOPP there are visible some hills beside the formation of large 2D sheets (few nm height and few microns length/width), with heights up to 30 nm.
3.2. Fractal Analysis
From the correlation function method combined with variable length scale method the following fractal characteristics could be summarized. For sample (1) the bare substrate was evidenced (; 2.20), the porphyrin powder being organized in fractal structures of different values: 2.45–2.46, for the correlations between the much closed particles (close “neighbors”), and 2.60–2.81, for long distance correlations between porphyrin particles (grains). The structure is self-similar on a rather large domain of self-similarity. Regarding sample (2) it is also revealed that the bare substrate is visible in fractal analysis at the scale of and microns ( and 2.07, resp.). The porphyrin powder exhibits low distance correlations between particles and low fractal dimensions (2.22–2.30). The image at the scale of microns shows a diffuse structure with fractal dimension of 2.22. Medium and long distance correlations are characterized by fractal dimensions on the range of 2.45–2.57. Sample (3) has a fractal dimension and 2.06 for the bare substrate at the scale of microns while the porphyrin is characterized by self-similar structures of low fractal dimension 2.22–2.37 at low scales of microns (not well agglomerated). A fractal dimension of 2.32 is kept at larger scales on which is superimposed a self-similar columnar structure with . In sample (4) self-similar agglomerations (aggregates-stacks) with low fractal dimensions of 2.23–2.30 on a large domain of self-similarity are obtained on which a structure with medium fractal dimension (2.51) is superimposed but with a low domain of self-similarity at middle scales. Self-similar columnar structures were obtained for sample (5), with different fractal dimensions, without correlations between structures, though there is a structure of low fractal dimension (2.26) on a large domain of self-similarity. In sample (6), besides the bare substrate (), there is an agglomerated self-similar structure with low fractal dimension 2.29–2.37 on a large domain of similarity. A diffuse but self-similar structure, scattered, was also observed (). The agglomerations are self-correlated with big fractal dimensions, 2.70.
From all the collected data a profile for the aggregation tendencies could be set as follow: TMHAPP < THAPP < MHTPP < TMDOPP < TMDPP < TRMDOPP, the last compound being the most susceptible to form larger aggregates.
3.3. Cytotoxicity Tests
The investigated porphyrinic samples were further tested for their biological response, in two cells interaction tests, eventually targeting the cancer therapy. The results are different, but complementary, as follows: significant influence of aggregation process in the MTS assay results (Figure 4) and constant low impact behavior in LDH release tests (Figure 5).
3.3.1. MTS Assay
The short-term dark toxicity of the porphyrinic compounds was evaluated on standard T lymphocytes cell lines Jurkat type. For these biosamples was assessed the cell proliferation from the test of [3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] reduction, the cells being cultured in 96-well plates in RPMI medium for 3 hours at 37°C in an atmosphere containing 5% CO2 with the porphyrins in DMSO added in different concentrations. The targeted characteristics of porphyrins act invariably for the change of the solvent from water to DMSO.
Also, because the aggregation behavior is largely related to the solvents, the incompatible ones with biological experiments were from start eliminated. Water and DMSO proved to be suitable for this type of studies.
A direct, linear relation between AFM and biological experiments is revealed by the MTS assay studies, establishing a perfect match with the aggregation tendencies in porphyrins:
It is observed that, at incipient values of compound concentration, the response in terms of the cell viability remains between restraint borders. Increasing the concentration of added porphyrin solution, the influence in the cell response scattered the compounds behavior; at maximum used concentration the order in response is the same as in the tendency of aggregation scale. The differences in the values of optical density for the MTS release after 3 hours are 5 times bigger for the TRMDOPP than for the TMHAPP. This implies that the porphyrin with lower aggregation tendency behave less aggressive in bio environment.
3.3.2. LDH Release
The cell viability/membrane integrity evaluation was deduced from the lactate dehydrogenase (LDH) release test.
We highlighted that the investigated porphyrinic structures might interfere with cellular LDH particularly after 24 h incubation, but this issue has to be further investigated; anyway, from cytotoxicity point of view the different aggregation tendencies are not a significant factor in this kind of tests, all the registered values being concentrated in a restraint domain, at lower and increased concentrations. As displayed in Figure 4, in spite of their significant different agglomeration tendencies, the influence on the integrity of cell membrane could be qualified as neutral. The evaluation of the amount of LDH release, as key index for the permeabilization of plasma membrane in the presence of the porphyrinic samples, leads to the observation that they are nontoxic and, more importantly, independent of the aggregation degree.
Biology tests on these particular compounds are the essential departure milestone in revealing that they localize in the malignant cells and they have a reduced unwanted activity in the absence of laser irradiation.
AB3 asymmetric meso-porphyrins have been studied by atomic force microscopy in order to evaluate their morphology and establish a direct visual pattern of their tendencies of aggregation; AFM images were further analyzed based on fractal theory with two mathematical methods, namely, the correlation function method and the variable length scale method. From AFM and fractal investigations the following order of the aggregation tendencies of the studied compounds has been established: TMHAPP < THAPP < MHTPP < TMDOPP < TMDPP < TRMDOPP. This order is maintained in the basic biological experiment result involved in the assessment of cell metabolic activity, targeting cancer treatment; the larger the aggregates the lower the power to interfere in the cells activity. Instead, the aggregation degree of porphyrins remains with no consequences when involved in dark-cytotoxicity tests, all proving the same low toxic behavior against tested cells.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
This research was supported by Project MNT-7-030/2010 of the Romanian Ministry of Education and Research. Support from the EU (ERDF) and Romanian Government infrastructure POS-CCE O 2.2.1 Project INFRANANOCHEM no. 19/2009 is gratefully acknowledged (AFM equipment).
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