Combined Application of 17-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice
Figure 7
Quantification
of VEGF protein release of alveolar cells type II treated for 48 h with E2-8 M and P-8 M alone or in combination determined by
ELISA. Note that corresponding with results obtained by gene
expression analysis (Figure 6) VEGF protein is increased in alveolar type II
cell cultures only by combined treatment with E2 and P. Pretreatment with ICI/RU 486 abrogated
this effect. control versus E2/P-8 M.
