Combined Application of 17𝛽-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice

Table 1

Primer sequences for mRNA detection of the different gene products.

Gen	Forward	Reverse	bp	AT
VEGF^*	cca cgt cag aga gca aca tca	tca ttc tct cta tgt gct ggc ttt	71	60
SP-B	cca cct cct cac aaa gat gac	ttg ggg tta atc tgg ctc tgg	174	60
SP-C	atg gac atg agt agc aaa gag gt	cac gat gag aag gcg ttt gag	119	60
ER-a	cgt gtg caa tga cta tgc ctc	ttt cat cat gcc cacttcgtaa	199	62
ER-b	ctg tga tga act acagtg ttc cc	gca gtg ggt ggc taa agg a	124	62
PR	cca act tca caa aac ttc tcg aca	ggc agc aat aac ttc aga cat ca	127	64
HPRT	gct ggt gaa aaggac ctc t	cac agg act aga aca cct gc	248	62

^*recognizes transcript variants VEGF 120, 144, and 164.