Fecal Microbiota and Diet of Children with Chronic Constipation

Table 1

Primers of groups in real-time polymerase chain reaction.

Group or species	Oligonucleotide sequence (5′-3′)	Verification media	Reference
Eubacteria (total bacteria)	UniF340: ACTCCTACGGGAGGCAGCAGT	11 strains of Eubacteria	[23, 24]
	UniR514: ATTACCGCGGCTGCTGGC
Lactobacillus spp.	gLactoF: TGGAAACAGRTGCTAATACCG	21 strains of Lactobacillus	[25]
	gLactoR: GTCCATTGTGGAAGATTCCC
	LacAcR: GCGGAAACCTCCCAACA
Bifidobacterium spp.	gBifidF: CTCCTGGAAACGGGTGG	11 strains of Bifidobacterium	[26]
	gBifidR: GGTGTTCTTCCCGATATCTACA
	BiLonR_2: TACCCGTCGAAGCCAC