Table of Contents
International Journal of Proteomics
Volume 2011, Article ID 145010, 8 pages
http://dx.doi.org/10.1155/2011/145010
Research Article

Comprehensive Proteomic Profiling of Aldehyde Dehydrogenases in Lung Adenocarcinoma Cell Lines

1Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5-C800, Seattle, WA 98109, USA
2University of Michigan Comprehensive Cancer Center, 8D17 300 NIB, University of Michigan, Ann Arbor, MI 48109, USA
3Department of Surgery, University of Michigan, 1150 West Medical Center Drive, Room A510A MSRB-1, Ann Arbor, MI 48109, USA

Received 1 July 2011; Accepted 14 August 2011

Academic Editor: Tadashi Kondo

Copyright © 2011 Qing Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have explored the potential of proteomic profiling to contribute to the delineation of the range of expression and subcellular localization of aldehyde dehydrogenases (ALDHs) in lung adenocarcinoma. In-depth quantitative proteomics was applied to 40 lung adenocarcinoma cell lines resulting in the identification of the known members of the ALDH family. Substantial heterogeneity in the level and occurrence of ALDHs in total lysates and on the cell surface and in their release into the culture media was observed based on mass spectrometry counts. A distinct pattern of expression of ALDHs was observed in cells exhibiting epithelial features relative to cells exhibiting mesenchymal features. Strikingly elevated levels of ALDH1A1 were observed in two cell lines. We also report on the occurrence of an immune response to ALDH1A1 in lung cancer.