Table of Contents
International Journal of Proteomics
Volume 2011 (2011), Article ID 214715, 18 pages
http://dx.doi.org/10.1155/2011/214715
Research Article

Urine Glycoprotein Profile Reveals Novel Markers for Chronic Kidney Disease

1Division of Nephrology, University of Michigan, Ann Arbor, MI 48105, USA
2Department of Pathology, University of Michigan, Ann Arbor, MI 48105, USA
3Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48105, USA
4Department of Computational Medicine and Biology, University of Michigan, Ann Arbor, MI 48105, USA
5Department of Surgery, University of Michigan, Ann Arbor, MI 48105, USA

Received 30 June 2011; Accepted 30 July 2011

Academic Editor: David E. Misek

Copyright © 2011 Anuradha Vivekanandan-Giri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chronic kidney disease (CKD) is a significant public health problem, and progression to end-stage renal disease leads to dramatic increases in morbidity and mortality. The mechanisms underlying progression of disease are poorly defined, and current noninvasive markers incompletely correlate with disease progression. Therefore, there is a great need for discovering novel markers for CKD. We utilized a glycoproteomic profiling approach to test the hypothesis that the urinary glycoproteome profile from subjects with CKD would be distinct from healthy controls. N-linked glycoproteins were isolated and enriched from the urine of healthy controls and subjects with CKD. This strategy identified several differentially expressed proteins in CKD, including a diverse array of proteins with endopeptidase inhibitor activity, protein binding functions, and acute-phase/immune-stress response activity supporting the proposal that inflammation may play a central role in CKD. Additionally, several of these proteins have been previously linked to kidney disease implicating a mechanistic role in disease pathogenesis. Collectively, our observations suggest that the human urinary glycoproteome may serve as a discovery source for novel mechanism-based biomarkers of CKD.