Table of Contents
International Journal of Proteomics
Volume 2011 (2011), Article ID 502845, 8 pages
http://dx.doi.org/10.1155/2011/502845
Research Article

Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating

1Clinical Proteomics Research Center, Chiba University Hospital, Chiba, Japan
2R&D Department, Nittobo Medical Co., Ltd., Fukushima, Koriyama City 963-8061, Japan
3Department of Clinical Laboratory, Chiba University Hospital, Chiba, Japan
4Department of Molecular Diagnosis (F8), Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan

Received 1 June 2011; Accepted 5 July 2011

Academic Editor: David E. Misek

Copyright © 2011 Iwao Kiyokawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

One possible factor determining recovery of trace amount of protein biomarker candidates during proteome analyses could be adsorption on urine tubes. This issue, however, has not been well addressed so far. Recently, a new technical device of surface coating by poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)) (poly(MPC-co-BMA)) has been developed mainly to prevent the adsorption of plasma proteins. We assessed whether conventionally used urine tubes adsorb trace amount of urinary proteins and, if any, whether the surface coating by poly(MPC-co-BMA) can minimize the adsorption. Proteinuric urine samples were kept in poly(MPC-co-BMA)-coated and noncoated urine tubes for 15 min and possibly adsorbed proteins and/or peptides onto urine tubes were analyzed by SDS-PAGE, 2-DE, and the MALDI-TOF MS. It was found that a number of proteins and/or peptides adsorb on the conventionally used urine tubes and that surface coating by poly(MPC-co-BMA) can minimize the adsorption without any significant effects on routine urinalysis test results. Although it remains to be clarified to what extent the protein adsorption can modify the results of urinary proteome analyses, one has to consider this possible adsorption of urinary proteins when searching for trace amounts of protein biomarkers in urine.