Table of Contents
International Journal of Proteomics
Volume 2012, Article ID 123076, 12 pages
Research Article

A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions

1Biological Sciences Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, WA 99352, USA
2Biomedical Sciences Graduate Program, University of California San Diego, 9500 Gilman Dive, La Jolla, CA 92063, USA
3Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA

Received 16 March 2012; Accepted 6 June 2012

Academic Editor: Gary B. Smejkal

Copyright © 2012 Roslyn N. Brown et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1 shows a 1D SDS-PAGE analysis of subcellular fractions of WT and flagellin mutant. Supplemental Figure 2 is a Venn Diagram illustrating the overlap in protein identifications among the three growth conditions. Seven supplemental data tables provided include (1) a preliminary MS analysis of Salmonella subcellular fractions and rationale for flagellin mutants, (2) a comparison of the PERI fractions in WT versus flagellin mutant, (3) K-means clustering of Z-transformed protein abundances to determine primary protein localization, (4) analysis of IM-colocalized CYT proteins, (5) subcellular localization of hypothetical and uncharacterized proteins, (6) moonlighting candidate proteins, and (7) analysis of protein abundance changes under different growth conditions.

  1. Supplementary Figure 1
  2. Supplementary Figure 2
  3. Supplementary Table 1
  4. Supplementary Table 2
  5. Supplementary Table 3
  6. Supplementary Table 4
  7. Supplementary Table 5
  8. Supplementary Table 6