Table 2: Media and incubation conditions used for enumeration of representative microbes in the simulated human colon.

Microbial groupMediumIncubation conditions and timeColonies formed

Total aerobesBrain heart infusion agarAerobic, , 24 hoursWhite
Total anaerobesBrain heart infusion agarAnaerobic, , 72 hoursWhite
Escherichia coliMc Conkey agarAerobic, , 24 hoursRed-purple
Staphylococcus sp.Mannitol Salt agarAerobic, , 48 hoursWhite with yellow/purple zone
Lactobacillussp. Rogosa agarAnaerobic, , 72 hoursWhite