Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization

Table 3

Residual crude protein and ash content in decalcified shrimp shells and raw chitin flakes during purification by a two-step biological process.

Biological process	Crude protein; (%, w/w)	Ash content; (%, w/w)
First step: L.  pentosus L7 fermentation
24 hours	26.3±1.2	3.8±0.4
48 hours	23.2±2.5	1.4±0.5
Second step: deproteinization with B.  thuringiensis SA
24 hours	16.4±2.5	1.5±0.4
48 hours	8.0±1.2	1.5±0.4
72 hours	3.8±1.3	1.7±0.4