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International Journal of Polymer Science
Volume 2014, Article ID 236259, 6 pages
Research Article

Enhancement of VEGF on Axial Vascularization of Nano-HA/Collagen/PLA Composites: A Histomorphometric Study on Rabbits

1Department of Orthopaedic Surgery, Peking Union Medical College Hospital, No. 1, Shuaifuyuan Hutong, Beijing 100730, China
2Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, China

Received 28 January 2014; Accepted 6 March 2014; Published 31 March 2014

Academic Editor: Xiaoming Li

Copyright © 2014 Xiao Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to investigate whether the nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite is suitable to be compounded with VEGF to enhance the axial vascularization in vivo. Thirty rabbits were divided into 2 groups of 15 animals each. In control group, a nHAC/PLA scaffold slice was vascularized axially by an inserted ligated femoral arteriovenous (AV) bundle in the animal. In experimental group, a slice compounded with VEGF gel was applied. The rabbits were sacrificed at 2 weeks, 6 weeks, and 10 weeks after surgery; the specimens of scaffold slices underwent histomorphometric examination; analysis of the microvessel density (MVD) of both groups was done. The combination with VEGF (Group B) did not enhance the vascularization in early phase (2 and 6 weeks, ) but worked in later phase (10 weeks, ). The data of the experiment demonstrated the suitability of the nHAC/PLA composite as carrier for the growth factor VEGF, enabling its sustained release in bioactive form with enough binding efficacy.