Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice

Figure 1

Direct contact assay using P3HB, P3HBV, and polypropylene materials with mouse 3T3 cells. (a) The film was placed at the bottom of the indicated well followed by seeding 3T3 cells into the well with culture medium. Forty-eight hours later, the culture medium was discarded and the cells growing in the empty well (no film control) or on different films were fixed with 1% formalin solution. The cells were stained with 1% (w/v) crystal violet solution. (b) Six images in different fields were photographed from each sample, and the nuclei were counted using Image J software. The cell number in the control well was set at 100%. The bars represent the means of three independent experiments, and the error bars represent the standard deviation. Statistically significant difference between endotoxin-containing and endotoxin-free films.

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