Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice

Figure 2

Indirect contact assay using P3HB, P3HBV, and polypropylene materials with mouse 3T3 cells. (a) The film was soaked in a culture medium and gently shaken in an orbital shaker at 37°C for 24 h. Twenty-four hours later, the conditioned medium was harvested and used to culture 3T3 cells, which were incubated in a humidified 37°C CO₂ incubator for another 48 h. To observe the cell growth, cells in each well were fixed with 1% formalin solution and stained with 1% (w/v) crystal violet solution. (b) Six images in different fields were photographed from each sample, and the nuclei were counted using Image J software. The cell number in the control well was set as 100%. The bars represent the mean values of three independent experiments, and the error bars represent the standard deviation. Statistically significant difference between endotoxin-containing and endotoxin-free films.

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