Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice

Figure 3

In vivo bioabsorption of endotoxin-free P3HB and P3HBV films. The polymer films were cut to 1 cm × 1 cm pieces and were implanted into the subcutaneous region of the abdominal wall. After different time points (0.5, 1, 3, and 9 mo), the implanted films with the adjacent skin and muscular tissue were excised and subjected to tissue sectioning. (a) Through H&E staining, the sections were used for histologically evaluating the tissue response to different films. The scale bar represents 400 μm. (b) The remaining thickness of the indicated endotoxin-free film was measured using Image J software. Three slides from each tissue sample were observed and photographed. The film thickness was calculated using Image J software. The cell number in the control well was set at 100%. The plots represent the mean values of three independent experiments, and the error bars represent the standard deviation. Statistically significant difference between P3HB and P3HBV groups.

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