Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera

Table 1

Observed N-glycopeptides derived enzymatic digestion of lacquer stellacyanin.

Glycosylation	Trypsin-derived glycopeptides		Chymotrypsin-derived glycopeptides		N-glycan
Site	Peptide (mass)	Obs.	Peptide (mass)	Obs.	Mass	Composition^a
		888.12/2		919.19/2	1188.42	GlcNAc2 Hex3 Fuc1 Xyl1
		1070.85/2		1101.88/2	1553.56	GlcNAc3 Hex4 Fuc1 Xyl1
Asn 28	WASNK (604.29)	1144.03/2	ASNKTF (666.34)	1175.03/2	1699.61	GlcNAc3 Hex4 Fuc2 Xyl1
		1326.56/2		1357.50/2	2064.75	GlcNAc4 Hex5 Fuc2 Xyl1
		1399.88/2		1430.63/2	2210.80	GlcNAc4 Hex5 Fuc3 Xyl1
Asn 60	NYQSCNDTTPIASYNGTBBR (2290.93)	1154.68/3	QSCNDTTPIASY (1356.57)	1264.51/2	1188.42	GlcNAc2 Hex3 Fuc1 Xyl1
		1276.85/3		nd^b	1553.56	GlcNAc3 Hex4 Fuc1 Xyl1
		1324.24/3		1014.33/2	1699.61	GlcNAc3 Hex4 Fuc2 Xyl1
		1496.33/3		1184.74/3	2210.80	GlcNAc4 Hex5 Fuc3 Xyl1
		981.40/2		nd	1042.37	GlcNAc2 Hex3 Xyl1
		703.41/3		926.74/2	1188.42	GlcNAc2 Hex3 Fuc1 Xyl1
		776.31/3		nd	1407.50	GlcNAc3 Hex4 Xyl1
Asn 102	VHINVTVR (936.55)	825.35/3	HINVTV (681.38)	nd	1553.56	GlcNAc3 Hex4 Fuc1 Xyl1
		1237.38/2			1553.56	GlcNAc3 Hex4 Fuc1 Xyl1
		873.47/3		1182.87/2	1699.61	GlcNAc3 Hex4 Fuc2 Xyl1
		1310.40/2			1699.61	GlcNAc3 Hex4 Fuc2 Xyl1
		1044.45/3		1438.56/2	2210.80	GlcNAc4 Hex5 Fuc3 Xyl1

Monosaccharides represented as GlcNAc, N-acetylglucosamine; Hex, mannose or galactose; Fuc, fucose; Xyl, xylose.
nd: not detected.