Research Article
Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera
Table 1
Observed N-glycopeptides derived enzymatic digestion of lacquer stellacyanin.
| Glycosylation |
Trypsin-derived glycopeptides | Chymotrypsin-derived glycopeptides | N-glycan | Site | Peptide (mass) | Obs. | Peptide (mass) | Obs. | Mass | Compositiona |
| | | 888.12/2 | | 919.19/2 | 1188.42 | GlcNAc2 Hex3 Fuc1 Xyl1 | | | 1070.85/2 | | 1101.88/2 | 1553.56 | GlcNAc3 Hex4 Fuc1 Xyl1 | Asn 28 | WASNK (604.29) | 1144.03/2 | ASNKTF (666.34) | 1175.03/2 | 1699.61 | GlcNAc3 Hex4 Fuc2 Xyl1 | | | 1326.56/2 | | 1357.50/2 | 2064.75 | GlcNAc4 Hex5 Fuc2 Xyl1 | | | 1399.88/2 | | 1430.63/2 | 2210.80 | GlcNAc4 Hex5 Fuc3 Xyl1 |
| Asn 60 | NYQSCNDTTPIASYNGTBBR (2290.93) | 1154.68/3 | QSCNDTTPIASY (1356.57) | 1264.51/2 | 1188.42 | GlcNAc2 Hex3 Fuc1 Xyl1 | 1276.85/3 | ndb | 1553.56 | GlcNAc3 Hex4 Fuc1 Xyl1 | 1324.24/3 | 1014.33/2 | 1699.61 | GlcNAc3 Hex4 Fuc2 Xyl1 | 1496.33/3 | 1184.74/3 | 2210.80 | GlcNAc4 Hex5 Fuc3 Xyl1 |
| | | 981.40/2 | | nd | 1042.37 | GlcNAc2 Hex3 Xyl1 | | | 703.41/3 | | 926.74/2 | 1188.42 | GlcNAc2 Hex3 Fuc1 Xyl1 | | | 776.31/3 | | nd | 1407.50 | GlcNAc3 Hex4 Xyl1 | Asn 102 | VHINVTVR (936.55) | 825.35/3 | HINVTV (681.38) | nd | 1553.56 | GlcNAc3 Hex4 Fuc1 Xyl1 | | | 1237.38/2 | | | 1553.56 | GlcNAc3 Hex4 Fuc1 Xyl1 | | | 873.47/3 | | 1182.87/2 | 1699.61 | GlcNAc3 Hex4 Fuc2 Xyl1 | | | 1310.40/2 | | | 1699.61 | GlcNAc3 Hex4 Fuc2 Xyl1 | | | 1044.45/3 | | 1438.56/2 | 2210.80 | GlcNAc4 Hex5 Fuc3 Xyl1 |
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Monosaccharides represented as GlcNAc, N-acetylglucosamine; Hex, mannose or galactose; Fuc, fucose; Xyl, xylose. nd: not detected.
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