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International Journal of Polymer Science
Volume 2016 (2016), Article ID 3785036, 11 pages
http://dx.doi.org/10.1155/2016/3785036
Research Article

Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods

1School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China
2Jiangsu Yiming Biological Co., Ltd., Taizhou, Jiangsu 223001, China

Received 26 November 2015; Revised 15 April 2016; Accepted 9 May 2016

Academic Editor: Huining Xiao

Copyright © 2016 Maxiaoqi Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

ε-Polylysine (ε-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials. In the present work, we evaluated the ε-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorption conditions by single-factor test, response surface method, and orthogonal design. The complexes of resin and ε-PL were characterized by SEM and FITR. The results indicated that D155 resin had the best ε-PL adsorption performance and was selected for the separation and purification of ε-PL. The conditions for the static adsorption of ε-PL on D155 resin were optimized as follows: ε-PL solution 40 g/L, pH 8.5, resins 15 g/L, and absorption time 14 h. The adsorption efficiency of ε-PL under the optimal conditions was 96.84%. The ε-PL adsorbed on the D155 resin was easily desorbed with 0.4 mol/L HCl at 30°C in 10 h. The highest desorption efficiency was 97.57% and the overall recovery of ε-PL was 94.49% under the optimal conditions. The excellent ε-PL adsorption and desorption properties of D155 resin including high selectivity and adsorption capacity, easy desorption, and high stability make it a good candidate for the isolation of ε-PL from fermentation broths.