Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity

Figure 2

Representative ALP staining photographs after one or two weeks of culture: (a, e) FC-negative group, (b, f) FC-positive group, (c, g) FC-negative and BGP-positive group, and (d, h) FC-positive and BGP-positive group. ALP staining shows either a yellow or brown color. Although the typical pavement-like shape is observed at (a), (e), and (b), (f), on the whole, the cell morphology changes to a spindle-like cell shape (c, g, and d, h). Scale bar = 15 μm.