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International Journal of Polymer Science
Volume 2016, Article ID 7354396, 9 pages
Research Article

Cell Adhesion on Polycaprolactone Modified by Plasma Treatment

1Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, Slovenia
2Jozef Stefan International Postgraduate School, Jamova Cesta 39, 1000 Ljubljana, Slovenia
3National Institute of Biology, Večna Pot 111, 1000 Ljubljana, Slovenia
4International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India
5Department of Materials Science and Engineering, Technion-Israel Institute of Technology, De-Jur Building, Technion City, 3200003 Haifa, Israel
6School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, India
7School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala 686560, India

Received 19 October 2015; Revised 19 March 2016; Accepted 28 March 2016

Academic Editor: Anezka Lengalova

Copyright © 2016 Nina Recek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have investigated the influence of various plasma treatments of electrospun polycaprolactone (PCL) scaffolds on the adhesion and proliferation of human umbilical endothelial cells (HUVEC). The PCL scaffolds were treated in plasmas created in O2, NH3 or SO2 gas at identical conditions. Surface functionalization of plasma-treated samples was determined using X-ray photoelectron spectroscopy. Cell adhesion and morphology were investigated by scanning electron microscopy and the influence of plasma treatment on cell adhesion and viability was evaluated with cell viability assay (MTT assay). The results showed the highest metabolic activity of HUVEC on PCL samples treated with O2 and NH3 plasma. Accordingly, the cells reflected the best adhesion and morphology on O2 and NH3 plasma-treated PCL samples already at 3 h. Moreover, treatment with O2 and NH3 plasma even stimulated endothelial cell proliferation on PCL surfaces by 60% as measured at 24 h, showing significant improvement in endothelialization of this material. Contrarily, SO2 plasma appeared to be less promising in comparison with O2 and NH3 plasma; however, it was still better than without any plasma treatment. Thus, our results importantly contribute to the biocompatibility improvement of the PCL polymer, commonly used for scaffolds in tissue engineering.