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International Journal of Polymer Science
Volume 2016, Article ID 7697031, 17 pages
http://dx.doi.org/10.1155/2016/7697031
Review Article

Alginate: Current Use and Future Perspectives in Pharmaceutical and Biomedical Applications

1Department of Pharmaceutical Technology, Medical University of Białystok, Mickiewicza 2c, 15-222 Białystok, Poland
2Department of Microbioanalytics, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland

Received 26 September 2016; Revised 16 November 2016; Accepted 5 December 2016

Academic Editor: Muhammet U. Kahveci

Copyright © 2016 Marta Szekalska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. A. Repka and A. Singh, “Alginic acid,” in Handbook of Pharmaceutical Excipients, pp. 20–22, Pharmaceutical Press, London, UK, 6th edition, 2009. View at Google Scholar
  2. K. N. Sachan, S. Pushkar, A. Jha, and A. Bhattcharya, “Sodium alginate: the wonder polymer for controlled drug delivery,” Journal of Pharmacy Research, vol. 2, no. 8, pp. 1191–1199, 2009. View at Google Scholar
  3. U. Remminghorst and B. H. A. Rehm, “Bacterial alginates: from biosynthesis to applications,” Biotechnology Letters, vol. 28, no. 21, pp. 1701–1712, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. C. G. Cable, “Sodium alginate,” in Handbook of Pharmaceutical Excipients, pp. 622–624, Pharmaceutical Press, London, UK, 6th edition, 2009. View at Google Scholar
  5. The United States Pharmacopeia, “The United States pharmacopeial convention,” USP 34-NF 29, The United States Pharmacopeial Convention, Rockville, Md, USA, 2011. View at Google Scholar
  6. Council of Europe, The European Pharmacopoeia 7.0, Council of Europe, Strasbourg, France, 2011.
  7. S. A. Shah and D. Thassu, “Ammonium alginate,” in Handbook of Pharmaceutical Excipients, p. 41, Pharmaceutical Press, London, UK, 6th edition, 2009. View at Google Scholar
  8. C. G. Gable, “Calcium alginate,” in Handbook of Pharmaceutical Excipients, pp. 83–85, Pharmaceutical Press, London, UK, 6th edition, 2009. View at Google Scholar
  9. R. G. Nause, R. D. Reddy, and J. L. P. Soh, “Propylene glycol alginate,” in Handbook of Pharmaceutical Excipients, pp. 594–595, Pharmaceutical Press, London, UK, 6th edition, 2009. View at Google Scholar
  10. B. Wright, P. A. De Bank, K. A. Luetchford, F. R. Acosta, and C. J. Connon, “Oxidized alginate hydrogels as niche environments for corneal epithelial cells,” Journal of Biomedical Materials Research—Part A, vol. 102, no. 10, pp. 3393–3400, 2014. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Maiti, K. Singha, S. Ray, P. Dey, and B. Sa, “Adipic acid dihydrazide treated partially oxidized alginate beads for sustained oral delivery of flurbiprofen,” Pharmaceutical Development and Technology, vol. 14, no. 5, pp. 461–470, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. R. Mahou, R. R. H. Meier, L. H. Bühler, and C. Wandrey, “Alginate-poly(ethylene glycol) hybrid microspheres for primary cell microencapsulation,” Materials, vol. 7, no. 1, pp. 275–286, 2014. View at Publisher · View at Google Scholar · View at Scopus
  13. Ø. Arlov, G. Skjåk-Bræk, and A. M. Rokstad, “Sulfated alginate microspheres associate with factor H and dampen the inflammatory cytokine response,” Acta Biomaterialia, vol. 42, pp. 180–188, 2016. View at Publisher · View at Google Scholar
  14. R. J. Coleman, G. Lawrie, L. K. Lambert, M. Whittaker, K. S. Jack, and L. Grndahl, “Phosphorylation of alginate: synthesis, characterization, and evaluation of in vitro mineralization capacity,” Biomacromolecules, vol. 12, no. 4, pp. 889–897, 2011. View at Publisher · View at Google Scholar · View at Scopus
  15. R. Tripathi and B. Mishra, “Development and evaluation of sodium alginate-polyacrylamide graft-co-polymer-based stomach targeted hydrogels of famotidine,” AAPS PharmSciTech, vol. 13, no. 4, pp. 1091–1102, 2012. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Chang, “pH-sensitive starch-g-poly(acrylic acid)/sodium alginate hydrogels for controlled release of diclofenac sodium,” Iranian Polymer Journal, vol. 24, no. 2, pp. 161–169, 2015. View at Publisher · View at Google Scholar
  17. X. Wang, T. Hao, J. Qu, C. Wang, and H. Chen, “Synthesis of thermal polymerizable alginate-GMA hydrogel for cell encapsulation,” Journal of Nanomaterials, vol. 2015, Article ID 970619, 8 pages, 2015. View at Publisher · View at Google Scholar · View at Scopus
  18. M. Tian, B. Han, H. Tan, and C. You, “Preparation and characterization of galactosylated alginate-chitosan oligomer microcapsule for hepatocytes microencapsulation,” Carbohydrate Polymers, vol. 112, pp. 502–511, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. W. Pluemsab, Y. Fukazawa, T. Furuike, Y. Nodasaka, and N. Sakairi, “Cyclodextrin-linked alginate beads as supporting materials for Sphingomonas cloacae, a nonylphenol degrading bacteria,” Bioresource Technology, vol. 98, no. 11, pp. 2076–2081, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. H. Izawa, K. Kawakami, M. Sumita, Y. Tateyama, J. P. Hill, and K. Ariga, “β-Cyclodextrin-crosslinked alginate gel for patient-controlled drug delivery systems: regulation of host-guest interactions with mechanical stimuli,” Journal of Materials Chemistry B, vol. 1, no. 16, pp. 2155–2161, 2013. View at Publisher · View at Google Scholar · View at Scopus
  21. R. Hurteaux, F. Edwards-Lévy, D. Laurent-Maquin, and M.-C. Lévy, “Coating alginate microspheres with a serum albumin-alginate membrane: application to the encapsulation of a peptide,” European Journal of Pharmaceutical Sciences, vol. 24, no. 2-3, pp. 187–197, 2005. View at Publisher · View at Google Scholar · View at Scopus
  22. Clinical Trials (Internet), U.S. National Institutes of Health, https://clinicaltrials.gov/ct2/show/NCT01858584?term=magnesium+alginate&rank=1.
  23. D. Ummarino, E. Miele, M. Martinelli et al., “Effect of magnesium alginate plus simethicone on gastroesophageal reflux in infants,” Journal of Pediatric Gastroenterology and Nutrition, vol. 60, no. 2, pp. 230–235, 2015. View at Publisher · View at Google Scholar · View at Scopus
  24. Md. Jakaria, R. Zaman, M. Parvez et al., “Comparative study among the different formulation of antacid tablets by using acid-base neutralization reaction,” Global Journal of Pharmacology, vol. 9, no. 3, pp. 278–281, 2015. View at Publisher · View at Google Scholar
  25. A. De Ruigh, S. Roman, J. Chen, J. E. Pandolfino, and P. J. Kahrilas, “Gaviscon Double Action Liquid (antacid & alginate) is more effective than antacid in controlling post-prandial oesophageal acid exposure in GERD patients: a double-blind crossover study,” Alimentary Pharmacology and Therapeutics, vol. 40, no. 5, pp. 531–537, 2014. View at Publisher · View at Google Scholar · View at Scopus
  26. E. Thomas, A. Wade, G. Crawford, B. Jenner, N. Levinson, and J. Wilkinson, “Randomised clinical trial: relief of upper gastrointestinal symptoms by an acid pocket-targeting alginate–antacid (Gaviscon Double Action)—a double-blind, placebo-controlled, pilot study in gastro-oesophageal reflux disease,” Alimentary Pharmacology and Therapeutics, vol. 39, no. 6, pp. 595–602, 2014. View at Publisher · View at Google Scholar · View at Scopus
  27. Z. M. Rashaan, P. Krijnen, M. E. van den Akker- van Marle et al., “Clinical effectiveness, quality of life and cost-effectiveness of Flaminal® versus Flamazine® in the treatment of partial thickness burns: study protocol for a randomized controlled trial,” Trials, vol. 17, article122, 9 pages, 2016. View at Publisher · View at Google Scholar
  28. E. Caló and V. V. Khutoryanskiy, “Biomedical applications of hydrogels: a review of patents and commercial products,” European Polymer Journal, vol. 65, pp. 252–267, 2015. View at Publisher · View at Google Scholar · View at Scopus
  29. C. Sussman and B. Bates-Jensen, “Management of the wound environment with dressings and topical agents,” in Wound Care a Collaborative Practice Manual for Health Professionals, p. 254, Lippincott Williams & Wilkins, 3rd edition, 2007. View at Google Scholar
  30. S. Carella, M. Maruccia, P. Fino, and M. G. Onesti, “An atypical case of Henoch-Shönlein purpura in a young patient: treatment of the skin lesions with hyaluronic acid-based dressings,” In Vivo, vol. 27, no. 1, pp. 147–151, 2013. View at Google Scholar · View at Scopus
  31. E. Ausili, V. Paolucci, S. Triarico et al., “Treatment of pressure sores in spina bifida patients with calcium alginate and foam dressings,” European Review for Medical and Pharmacological Sciences, vol. 17, no. 12, pp. 1642–1647, 2013. View at Google Scholar · View at Scopus
  32. S. Bale, N. Baker, H. Crook, A. Rayman, G. Rayman, and K. G. Harding, “Exploring the use of an alginate dressing for diabetic foot ulcers,” Journal of Wound Care, vol. 10, no. 3, pp. 81–84, 2001. View at Publisher · View at Google Scholar · View at Scopus
  33. M. Porter and J. Kelly, “Pressure ulcer treatment in a patient with spina bifida,” Nursing Standard, vol. 28, no. 35, pp. 60–69, 2014. View at Publisher · View at Google Scholar · View at Scopus
  34. E. J. Sohn, H. B. Ahn, M. S. Roh, W. Y. Ryu, and Y. H. Kwon, “Efficacy of temperature-sensitive guardix-sg for adhesiolysis in experimentally induced eyelid adhesion in rabbits,” Ophthalmic Plastic and Reconstructive Surgery, vol. 29, no. 6, pp. 458–463, 2013. View at Publisher · View at Google Scholar · View at Scopus
  35. S. O. Park, J. Han, K. W. Minn, and U. S. Jin, “Prevention of capsular contracture with Guardix-SG® after silicone implant insertion,” Aesthetic Plastic Surgery, vol. 37, no. 3, pp. 543–548, 2013. View at Publisher · View at Google Scholar · View at Scopus
  36. L. Abramowitz, G. H. Weyandt, B. Havlickova et al., “The diagnosis and management of haemorrhoidal disease from a global perspective,” Alimentary Pharmacology and Therapeutics, vol. 31, supplement 1, pp. 1–58, 2010. View at Google Scholar · View at Scopus
  37. E. Gruskin, B. A. Doll, F. W. Futrell, J. P. Schmitz, and J. O. Hollinger, “Demineralized bone matrix in bone repair: history and use,” Advanced Drug Delivery Reviews, vol. 64, no. 12, pp. 1063–1077, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. E. Al Machot, T. Hoffmann, K. Lorenz, I. Khalili, and B. Noack, “Clinical outcomes after treatment of periodontal intrabony defects with nanocrystalline hydroxyapatite (Ostim) or enamel matrix derivatives (Emdogain): a randomized controlled clinical trial,” BioMed Research International, vol. 2014, Article ID 786353, 9 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  39. X. Z. Yan, F. Rathe, C. Gilissen et al., “The effect of enamel matrix derivative (Emdogain®) on gene expression profiles of human primary alveolar bone cells,” Journal of Tissue Engineering and Regenerative Medicine, vol. 8, no. 6, pp. 463–472, 2014. View at Publisher · View at Google Scholar · View at Scopus
  40. A. Sculean, T. M. Auschill, N. Donos, M. Brecx, and N. B. Arweiler, “Effect of an enamel matrix protein derivative (Emdogain) on ex vivo dental plaque vitality,” Journal of Clinical Periodontology, vol. 28, no. 11, pp. 1074–1078, 2001. View at Publisher · View at Google Scholar · View at Scopus
  41. A. Barzegari and A. A. Saei, “An update to space biomedical research: tissue engineering in microgravity bioreactors,” BioImpacts, vol. 2, no. 1, pp. 23–32, 2012. View at Publisher · View at Google Scholar · View at Scopus
  42. S. Khan, A. Tøndervik, H. Sletta et al., “Overcoming drug resistance with alginate oligosaccharides able to potentiate the action of selected antibiotics,” Antimicrobial Agents and Chemotherapy, vol. 56, no. 10, pp. 5134–5141, 2012. View at Publisher · View at Google Scholar · View at Scopus
  43. M. F. Pritchard, L. C. Powell, G. E. Menzies et al., “A new class of safe oligosaccharide polymer therapy to modify the mucus barrier of chronic respiratory disease,” Molecular Pharmaceutics, vol. 13, no. 3, pp. 863–872, 2016. View at Publisher · View at Google Scholar
  44. L. C. Powell, M. F. Pritchard, C. Emanuel et al., “A nanoscale characterization of the interaction of a novel alginate oligomer with the cell surface and motility of Pseudomonas aeruginosa,” American Journal of Respiratory Cell and Molecular Biology, vol. 50, no. 3, pp. 483–492, 2014. View at Publisher · View at Google Scholar · View at Scopus
  45. X. L. Xin, M. Y. Geng, H. S. Guan, and Z. L. Li, “Study on the mechanism of inhibitory action of 911 on replication of HIV-1 in vitro,” Chinese Journal of Marine Drugs, vol. 19, no. 4, pp. 15–18, 2000. View at Google Scholar
  46. X. L. Xin, H. Ding, M. Y. Geng, P. F. Liang, Y. X. Li, and H. S. Guan, “Studies of the anti-AIDS effects of marine polysaccharide drug 911 and its related mechanisms of action,” Chinese Journal of Marine Drugs, vol. 19, no. 6, pp. 4–8, 2000. View at Google Scholar
  47. B. F. Jiang, X. F. Xu, L. Li, and W. Yuan, “Study on ‘911’ anti-HBV effect in HepG2.2.15 cells culture,” Modern Preventive Medicine, no. 30, pp. 517–518, 2003. View at Google Scholar
  48. Y.-L. Wu, J. Ai, J.-M. Zhao et al., “Sulfated polymannuroguluronate inhibits Tat-induced SLK cell adhesion via a novel binding site, a KKR spatial triad,” Acta Pharmacologica Sinica, vol. 32, no. 5, pp. 647–654, 2011. View at Publisher · View at Google Scholar · View at Scopus
  49. S. K. Tusi, L. Khalaj, G. Ashabi, M. Kiaei, and F. Khodagholi, “Alginate oligosaccharide protects against endoplasmic reticulum- and mitochondrial-mediated apoptotic cell death and oxidative stress,” Biomaterials, vol. 32, no. 23, pp. 5438–5458, 2011. View at Publisher · View at Google Scholar · View at Scopus
  50. R. Zhou, X.-Y. Shi, D.-C. Bi, W.-S. Fang, G.-B. Wei, and X. Xu, “Alginate-derived oligosaccharide inhibits neuroinflammation and promotes microglial phagocytosis of β-amyloid,” Marine Drugs, vol. 13, no. 9, pp. 5828–5846, 2015. View at Publisher · View at Google Scholar · View at Scopus
  51. V. Manigandan, R. Karthik, and R. Saravanan, “Marine carbohydrate based therapeutics for Alzheimer disease—mini review,” Journal of Neurology and Neuroscience, In press.
  52. J. Hu, M. Geng, J. Li et al., “Acidic oligosaccharide sugar chain, a marine-derived acidic oligosaccharide, inhibits the cytotoxicity and aggregation of amyloid beta protein,” Journal of Pharmacological Sciences, vol. 95, no. 2, pp. 248–255, 2004. View at Publisher · View at Google Scholar · View at Scopus
  53. S. Wang, J. Li, W. Xia, and M. Geng, “A marine-derived acidic oligosaccharide sugar chain specifically inhibits neuronal cell injury mediated by β-amyloid-induced astrocyte activation in vitro,” Neurological Research, vol. 29, no. 1, pp. 96–102, 2007. View at Publisher · View at Google Scholar · View at Scopus
  54. H. Ronghua, D. Yumin, and Y. Jianhong, “Preparation and in vitro anticoagulant activities of alginate sulfate and its quaterized derivatives,” Carbohydrate Polymers, vol. 52, no. 1, pp. 19–24, 2003. View at Publisher · View at Google Scholar · View at Scopus
  55. M. Xin, L. Ren, Y. Sun et al., “Anticoagulant and antithrombotic activities of low-molecular-weight propylene glycol alginate sodium sulfate (PSS),” European Journal of Medicinal Chemistry, vol. 114, pp. 33–40, 2016. View at Publisher · View at Google Scholar
  56. M. Falkeborg, L.-Z. Cheong, C. Gianfico et al., “Alginate oligosaccharides: enzymatic preparation and antioxidant property evaluation,” Food Chemistry, vol. 164, pp. 185–194, 2014. View at Publisher · View at Google Scholar · View at Scopus
  57. R. Zhou, X. Shi, Y. Gao, N. Cai, Z. Jiang, and X. Xu, “Anti-inflammatory activity of guluronate oligosaccharides obtained by oxidative degradation from alginate in lipopolysaccharide-activated murine macrophage RAW 264.7 cells,” Journal of Agricultural and Food Chemistry, vol. 63, no. 1, pp. 160–168, 2015. View at Publisher · View at Google Scholar · View at Scopus
  58. Q.-D. An, G.-L. Zhang, H.-T. Wu et al., “Alginate-deriving oligosaccharide production by alginase from newly isolated Flavobacterium sp. LXA and its potential application in protection against pathogens,” Journal of Applied Microbiology, vol. 106, no. 1, pp. 161–170, 2009. View at Publisher · View at Google Scholar · View at Scopus
  59. J. Ji, L. Wang, H. Wu, and H. Luan, “Bio-function summary of marine oligosaccharides,” International Journal of Biology, vol. 3, no. 1, pp. 74–86, 2011. View at Publisher · View at Google Scholar
  60. X. Hu, X. Jiang, H. Hwang, S. Liu, and H. Guan, “Antitumour activities of alginate-derived oligosaccharides and their sulphated substitution derivatives,” European Journal of Phycology, vol. 39, no. 1, pp. 67–71, 2004. View at Publisher · View at Google Scholar · View at Scopus
  61. X. Xu, X. Wu, Q. Wang et al., “Immunomodulatory effects of alginate oligosaccharides on murine macrophage RAW264.7 cells and their structure-activity relationships,” Journal of Agricultural and Food Chemistry, vol. 62, no. 14, pp. 3168–3176, 2014. View at Publisher · View at Google Scholar · View at Scopus
  62. X. Xu, D. Bi, X. Wu et al., “Unsaturated guluronate oligosaccharide enhances the antibacterial activities of macrophages,” FASEB Journal, vol. 28, no. 6, pp. 2645–2654, 2014. View at Publisher · View at Google Scholar · View at Scopus
  63. Y. Wang, F. Han, B. Hu, J. Li, and W. Yu, “In vivo prebiotic properties of alginate oligosaccharides prepared through enzymatic hydrolysis of alginate,” Nutrition Research, vol. 26, no. 11, pp. 597–603, 2006. View at Publisher · View at Google Scholar · View at Scopus
  64. S. Terakado, M. Ueno, Y. Tamura et al., “Sodium alginate oligosaccharides attenuate hypertension and associated kidney damage in Dahl salt-sensitive rats fed a high-salt diet,” Clinical and Experimental Hypertension, vol. 34, no. 2, pp. 99–106, 2012. View at Publisher · View at Google Scholar · View at Scopus
  65. T. Chaki, N. Kajimoto, H. Ogawa, T. Baba, and N. Hiura, “Metabolism and calcium antagonism of sodium alginate oligosaccharides,” Bioscience, Biotechnology and Biochemistry, vol. 71, no. 8, pp. 1819–1825, 2007. View at Publisher · View at Google Scholar · View at Scopus
  66. C. Moriya, Y. Shida, Y. Yamane et al., “Subcutaneous administration of sodium alginate oligosaccharides prevents salt-induced hypertension in dahl salt-sensitive rats,” Clinical and Experimental Hypertension, vol. 35, no. 8, pp. 607–613, 2013. View at Publisher · View at Google Scholar · View at Scopus
  67. C. K. Kuo and P. X. Ma, “Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties,” Biomaterials, vol. 22, no. 6, pp. 511–521, 2001. View at Publisher · View at Google Scholar · View at Scopus
  68. K. I. Draget, G. Skjåk Bræk, and O. Smidsrød, “Alginic acid gels: the effect of alginate chemical composition and molecular weight,” Carbohydrate Polymers, vol. 25, no. 1, pp. 31–38, 1994. View at Publisher · View at Google Scholar · View at Scopus
  69. D. Brault, A. Heyraud, V. Lognone, and M. Roussel, “Methods for obtaining oligomannuronates and guluronates, products obtained and use thereof,” Patent WO03099870, 2003.
  70. T. Shimokawa, S. Yoshida, T. Takeuchi, K. Murata, T. Ishii, and I. Kusakabe, “Preparation of two series of oligo-guluronic acids from sodium alginate by acid hydrolysis and enzymatic degradation,” Bioscience, Biotechnology and Biochemistry, vol. 60, no. 9, pp. 1532–1534, 1996. View at Publisher · View at Google Scholar · View at Scopus
  71. K. I. Draget, M. K. Simensen, E. Onsøyen, and O. Smidsrød, “Gel strength of Ca-limited alginate gels made in situ,” Hydrobiologia, vol. 260-261, no. 1, pp. 563–565, 1993. View at Publisher · View at Google Scholar · View at Scopus
  72. S. Fu, A. Thacker, D. M. Sperger et al., “Relevance of rheological properties of sodium alginate in solution to calcium alginate gel properties,” AAPS PharmSciTech, vol. 12, no. 2, pp. 453–460, 2011. View at Publisher · View at Google Scholar · View at Scopus
  73. B. B. Crow and K. D. Nelson, “Release of bovine serum albumin from a hydrogel-cored biodegradable polymer fiber,” Biopolymers, vol. 81, no. 6, pp. 419–427, 2006. View at Publisher · View at Google Scholar · View at Scopus
  74. A. D. Augst, H. J. Kong, and D. J. Mooney, “Alginate hydrogels as biomaterials,” Macromolecular Bioscience, vol. 6, no. 8, pp. 623–633, 2006. View at Publisher · View at Google Scholar · View at Scopus
  75. Y. Zhao, W. Shen, Z. Chen, and T. Wu, “Freeze-thaw induced gelation of alginates,” Carbohydrate Polymers, vol. 148, pp. 45–51, 2016. View at Publisher · View at Google Scholar
  76. W. R. Gombotz and S. F. Wee, “Protein release from alginate matrices,” Advanced Drug Delivery Reviews, vol. 31, no. 3, pp. 267–285, 1998. View at Publisher · View at Google Scholar · View at Scopus
  77. T. E. Jørgensen, M. Sletmoen, K. I. Draget, and B. T. Stokke, “Influence of oligoguluronates on alginate gelation, kinetics, and polymer organization,” Biomacromolecules, vol. 8, no. 8, pp. 2388–2397, 2007. View at Publisher · View at Google Scholar · View at Scopus
  78. B. Niekraszewicz and A. Niekraszewicz, “The structure of alginate, chitin and chitosan fibres,” in Handbook of Textile Fibre Structure, pp. 266–304, Woodhead Publishing Limited, Cambridge, UK, 2009. View at Google Scholar
  79. E. A. Wahl, F. A. Fierro, T. R. Peavy et al., “In vitro evaluation of scaffolds for the delivery of mesenchymal stem cells to wounds,” BioMed Research International, vol. 2015, Article ID 108571, 14 pages, 2015. View at Publisher · View at Google Scholar · View at Scopus
  80. J. W. Doyle, T. P. Roth, R. M. Smith, Y.-Q. Li, and R. M. Dunn, “Effect of calcium alginate on cellular wound healing processes modeled in vitro,” Journal of Biomedical Materials Research, vol. 32, no. 4, pp. 561–568, 1996. View at Publisher · View at Google Scholar · View at Scopus
  81. S. Thomas, “Alginate dressings in surgery and wound management—Part 1,” Journal of Wound Care, vol. 9, no. 2, pp. 56–60, 2000. View at Publisher · View at Google Scholar · View at Scopus
  82. H. Almeida, M. H. Amaral, P. Lobão, and J. M. S. Lobo, “In situ gelling systems: a strategy to improve the bioavailability of ophthalmic pharmaceutical formulations,” Drug Discovery Today, vol. 19, no. 4, pp. 400–412, 2014. View at Publisher · View at Google Scholar · View at Scopus
  83. A. Michael Rajesh, S. A. Bhatt, H. Brahmbhatt, P. S. Anand, and K. M. Popat, “Taste masking of ciprofloxacin by ion-exchange resin and sustain release at gastric-intestinal through interpenetrating polymer network,” Asian Journal of Pharmaceutical Sciences, vol. 10, no. 4, pp. 331–340, 2014. View at Publisher · View at Google Scholar · View at Scopus
  84. M. Jelvehgari, L. Barghi, and F. Barghi, “Preparation of chlorpheniramine maleate-loaded alginate/chitosan particulate systems by the ionic gelation method for taste masking,” Jundishapur Journal of Natural Pharmaceutical Products, vol. 9, no. 1, pp. 39–48, 2014. View at Publisher · View at Google Scholar · View at Scopus
  85. P. Ciosek, M. Wesoły, M. Zabadaj et al., “Towards flow-through/flow injection electronic tongue for the analysis of pharmaceuticals,” Sensors and Actuators, B: Chemical, vol. 207, pp. 1087–1094, 2015. View at Publisher · View at Google Scholar · View at Scopus
  86. K. Y. Lee and D. J. Mooney, “Alginate: properties and biomedical applications,” Progress in Polymer Science, vol. 37, no. 1, pp. 106–126, 2012. View at Publisher · View at Google Scholar
  87. H. H. Tønnesen and J. Karlsen, “Alginate in drug delivery systems,” Drug Development and Industrial Pharmacy, vol. 28, no. 6, pp. 621–630, 2002. View at Publisher · View at Google Scholar · View at Scopus
  88. A. L. Ching, C. V. Liew, L. W. Chan, and P. W. S. Heng, “Modifying matrix micro-environmental pH to achieve sustained drug release from highly laminating alginate matrices,” European Journal of Pharmaceutical Sciences, vol. 33, no. 4-5, pp. 361–370, 2008. View at Publisher · View at Google Scholar · View at Scopus
  89. J. Sun and H. Tan, “Alginate-based biomaterials for regenerative medicine applications,” Materials, vol. 6, no. 4, pp. 1285–1309, 2013. View at Publisher · View at Google Scholar · View at Scopus
  90. E. García-Gareta, N. Ravindran, V. Sharma, S. Samizadeh, and J. F. Dye, “A novel multiparameter in vitro model of three-dimensional cell ingress into scaffolds for dermal reconstruction to predict in vivo outcome,” BioResearch Open Access, vol. 2, no. 6, pp. 412–420, 2013. View at Publisher · View at Google Scholar
  91. W. Zhang and X. He, “Microencapsulating and banking living cells for cell-based medicine,” Journal of Healthcare Engineering, vol. 2, no. 4, pp. 427–446, 2011. View at Publisher · View at Google Scholar · View at Scopus
  92. R. Calafiore and G. Basta, “Clinical application of microencapsulated islets: actual prospectives on progress and challenges,” Advanced Drug Delivery Reviews, vol. 67-68, pp. 84–92, 2014. View at Publisher · View at Google Scholar · View at Scopus
  93. S. V. Hegarty, G. W. O'Keeffe, and A. M. Sullivan, “Neurotrophic factors: from neurodevelopmental regulators to novel therapies for Parkinson’s disease,” Neural Regeneration Research, vol. 9, no. 19, pp. 1708–1711, 2014. View at Publisher · View at Google Scholar · View at Scopus
  94. K. E. Haugstad, A. G. Håti, C. T. Nordgård et al., “Direct determination of chitosan—mucin interactions using a single-molecule strategy: comparison to alginate—mucin interactions,” Polymers, vol. 7, no. 2, pp. 161–185, 2015. View at Publisher · View at Google Scholar · View at Scopus
  95. G. Mythri, K. Kavitha, M. R. Kumar, and S. D. Jagadeesh Singh, “Novel mucoadhesive polymers—a review,” Journal of Applied Pharmaceutical Science, vol. 1, no. 8, pp. 37–42, 2011. View at Google Scholar · View at Scopus
  96. C. Taylor, J. P. Pearson, K. I. Draget, P. W. Dettmar, and O. Smidsrød, “Rheological characterisation of mixed gels of mucin and alginate,” Carbohydrate Polymers, vol. 59, no. 2, pp. 189–195, 2005. View at Publisher · View at Google Scholar · View at Scopus
  97. F. Laffleur, “Mucoadhesive polymers for buccal drug delivery,” Drug Development and Industrial Pharmacy, vol. 40, no. 5, pp. 591–598, 2014. View at Publisher · View at Google Scholar · View at Scopus
  98. C. Juliano, E. Gavini, M. Cossu, M. C. Bonferoni, and P. Giunchedi, “Mucoadhesive alginate matrices containing sodium carboxymethyl starch for buccal delivery: in vitro and in vivo studies,” Journal of Drug Delivery Science and Technology, vol. 14, no. 2, pp. 159–163, 2004. View at Publisher · View at Google Scholar · View at Scopus
  99. M. J. Martín, A. C. Calpena, F. Fernández, M. Mallandrich, P. Gálvez, and B. Clares, “Development of alginate microspheres as nystatin carriers for oral mucosa drug delivery,” Carbohydrate Polymers, vol. 117, pp. 140–149, 2015. View at Publisher · View at Google Scholar · View at Scopus
  100. S. Haque, S. Md, J. K. Sahni, J. Ali, and S. Baboota, “Development and evaluation of brain targeted intranasal alginate nanoparticles for treatment of depression,” Journal of Psychiatric Research, vol. 48, no. 1, pp. 1–12, 2014. View at Publisher · View at Google Scholar · View at Scopus
  101. R. J. Garmise, H. F. Staats, and A. J. Hickey, “Novel dry powder preparations of whole inactivated influenza virus for nasal vaccination,” AAPS PharmSciTech, vol. 8, no. 4, pp. 2–10, 2007. View at Google Scholar · View at Scopus
  102. J. R. Costa, N. C. Silva, B. Sarmento, and M. Pintado, “Potential chitosan-coated alginate nanoparticles for ocular delivery of daptomycin,” European Journal of Clinical Microbiology & Infectious Diseases, vol. 34, no. 6, pp. 1255–1262, 2015. View at Publisher · View at Google Scholar · View at Scopus
  103. Y. O. Batyrbekov, D. Rakhimbaeva, K. Musabekov, and B. Zhubanov, “Alginate based microparticle drug delivery systems for the treatment of eye cancer,” MRS Proceedings, vol. 1209, Article ID 1209-YY03-04, 2009. View at Publisher · View at Google Scholar
  104. P. Diós, S. Nagy, S. Pál et al., “Preformulation studies and optimization of sodium alginate based floating drug delivery system for eradication of Helicobacter pylori,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 96, pp. 196–206, 2015. View at Publisher · View at Google Scholar · View at Scopus
  105. A. O. Adebisi, P. R. Laity, and B. R. Conway, “Formulation and evaluation of floating mucoadhesive alginate beads for targeting Helicobacter pylori,” Journal of Pharmacy and Pharmacology, vol. 67, no. 4, pp. 511–524, 2015. View at Publisher · View at Google Scholar · View at Scopus
  106. M. Szekalska, M. Wróblewska, K. Sosnowska, and K. Winnicka, “Influence of sodium alginate on hypoglycemic activity of metformin hydrochloride in the microspheres obtained by the spray drying,” International Journal of Polymer Science, vol. 2016, 12 pages, 2016. View at Publisher · View at Google Scholar
  107. M. Szekalska, A. Amelian, and K. Winnicka, “Alginate microspheres obtained by the spray drying technique as mucoadhesive carriers of ranitidine,” Acta Pharmaceutica, vol. 65, no. 1, pp. 15–27, 2015. View at Publisher · View at Google Scholar · View at Scopus
  108. M. Szekalska, K. Winnicka, A. Czajkowska-Kośnik, K. Sosnowska, and A. Amelian, “Evaluation of alginate microspheres with metronidazole obtained by the spray drying technique,” Acta Poloniae Pharmaceutica—Drug Research, vol. 72, no. 3, pp. 569–578, 2015. View at Google Scholar · View at Scopus
  109. M. J. Martín-Villena, F. Fernández-Campos, A. C. Calpena-Campmany, N. Bozal-de Febrer, M. A. Ruiz-Martínez, and B. Clares-Naveros, “Novel microparticulate systems for the vaginal delivery of nystatin: development and characterization,” Carbohydrate Polymers, vol. 94, no. 1, pp. 1–11, 2013. View at Publisher · View at Google Scholar · View at Scopus
  110. A. Khare, K. Grover, P. Pawar, and I. Singh, “Mucoadhesive polymers for enhancing retention in ocular drug delivery: a critical review,” Reviews of Adhesion and Adhesives, vol. 2, no. 4, pp. 467–502, 2014. View at Publisher · View at Google Scholar · View at Scopus
  111. P. Prinderre, C. Sauzet, and C. Fuxen, “Advances in gastro retentive drug-delivery systems,” Expert Opinion on Drug Delivery, vol. 8, no. 9, pp. 1189–1203, 2011. View at Publisher · View at Google Scholar · View at Scopus
  112. A. A. Kharia and A. K. Singhai, “Screening of most effective variables for development of gastroretentive mucoadhesive nanoparticles by Taguchi design,” ISRN Nanomaterials, vol. 2013, 8 pages, 2013. View at Publisher · View at Google Scholar
  113. O. Borges, J. Tavares, A. de Sousa, G. Borchard, H. E. Junginger, and A. Cordeiro-da-Silva, “Evaluation of the immune response following a short oral vaccination schedule with hepatitis B antigen encapsulated into alginate-coated chitosan nanoparticles,” European Journal of Pharmaceutical Sciences, vol. 32, no. 4-5, pp. 278–290, 2007. View at Publisher · View at Google Scholar · View at Scopus
  114. F. Sarei, N. M. Dounighi, H. Zolfagharian, P. Khaki, and S. M. Bidhendi, “Alginate nanoparticles as a promising adjuvant and vaccine delivery system,” Indian Journal of Pharmaceutical Sciences, vol. 75, no. 4, pp. 442–449, 2013. View at Publisher · View at Google Scholar · View at Scopus
  115. P. Nyvall, E. Corre, C. Boisset et al., “Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata,” Plant Physiology, vol. 133, no. 2, pp. 726–735, 2003. View at Publisher · View at Google Scholar · View at Scopus
  116. C. Campa, S. Holtan, N. Nilsen, T. M. Bjerkan, B. T. Stokke, and G. Skjåk-Bræk, “Biochemical analysis of the processive mechanism for epimerization of alginate by mannuronan C-5 epimerase AlgE4,” The Biochemical Journal, vol. 381, no. 1, pp. 155–164, 2004. View at Publisher · View at Google Scholar · View at Scopus
  117. J.-S. Yang, Y.-J. Xie, and W. He, “Research progress on chemical modification of alginate: a review,” Carbohydrate Polymers, vol. 84, no. 1, pp. 33–39, 2011. View at Publisher · View at Google Scholar
  118. S. N. Pawar and K. J. Edgar, “Alginate derivatization: a review of chemistry, properties and applications,” Biomaterials, vol. 33, no. 11, pp. 3279–3305, 2012. View at Publisher · View at Google Scholar · View at Scopus
  119. T. W. Wong, “Alginate graft copolymers and alginate-co-excipient physical mixture in oral drug delivery,” The Journal of Pharmacy and Pharmacology, vol. 63, no. 12, pp. 1497–1512, 2011. View at Publisher · View at Google Scholar · View at Scopus
  120. S. W. Hutcheson, H. Zhang, and M. Suvorov, “Carbohydrase systems of Saccharophagus degradans degrading marine complex polysaccharides,” Marine Drugs, vol. 9, no. 4, pp. 645–665, 2011. View at Publisher · View at Google Scholar · View at Scopus
  121. E. Broderick, H. Lyons, T. Pembroke, H. Byrne, B. Murray, and M. Hall, “The characterisation of a novel, covalently modified, amphiphilic alginate derivative, which retains gelling and non-toxic properties,” Journal of Colloid and Interface Science, vol. 298, no. 1, pp. 154–161, 2006. View at Publisher · View at Google Scholar · View at Scopus
  122. H.-R. Lin, K. C. Sung, and W.-J. Vong, “In situ gelling of alginate/Pluronic solutions for ophthalmic delivery of pilocarpine,” Biomacromolecules, vol. 5, no. 6, pp. 2358–2365, 2004. View at Publisher · View at Google Scholar · View at Scopus
  123. G. Coşkun, E. Karaca, M. Ozyurtlu, S. Özbek, A. Yermezler, and I. Cavuşoğlu, “Histological evaluation of wound healing performance of electrospun poly(vinyl alcohol)/sodium alginate as wound dressing in vivo,” Bio-Medical Materials and Engineering, vol. 24, no. 2, pp. 1527–1536, 2014. View at Publisher · View at Google Scholar · View at Scopus
  124. A. A. Chang, M. S. Reuther, K. K. Briggs et al., “In vivo implantation of tissue-engineered human nasal septal neocartilage constructs: a pilot study,” Otolaryngology—Head and Neck Surgery, vol. 146, no. 1, pp. 46–52, 2012. View at Publisher · View at Google Scholar · View at Scopus
  125. S. Vériter, J. Mergen, R.-M. Goebbels et al., “In vivo selection of biocompatible alginates for islet encapsulation and subcutaneous transplantation,” Tissue Engineering Part A, vol. 16, no. 5, pp. 1503–1513, 2010. View at Publisher · View at Google Scholar · View at Scopus
  126. A. Sosnik, “Alginate particles as platform for drug delivery by the oral route: state-of-the-art,” ISRN Pharmaceutics, vol. 2014, Article ID 926157, 17 pages, 2014. View at Publisher · View at Google Scholar
  127. Generally Recognized as Safe (Internet), “U.S. Food and Drug Administration,” http://www.fda.gov/Food/IngredientsPackagingLabeling/GRAS/SCOGS/ucm260857.htm.
  128. M. Otterlei, K. Østgaard, G. Skjåk-Bræk, O. Smidsrød, P. Soon-Shiong, and T. Espevik, “Induction of cytokine production from human monocytes stimulated with alginate,” The Journal of Immunotherapy, vol. 10, no. 4, pp. 286–291, 1991. View at Publisher · View at Google Scholar · View at Scopus
  129. M. Ménard, J. Dusseault, G. Langlois et al., “Role of protein contaminants in the immunogenicity of alginates,” Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 93, no. 2, pp. 333–340, 2010. View at Publisher · View at Google Scholar
  130. M. Greco, C. A. Sáez, M. T. Brown, and M. B. Bitonti, “A simple and effective method for high quality co-extraction of genomic DNA and total RNA from low biomass Ectocarpus siliculosus, the model brown alga,” PLoS ONE, vol. 9, no. 5, Article ID e96470, 2014. View at Publisher · View at Google Scholar
  131. L. Corvaglia, C. Monari, S. Martini, A. Aceti, and G. Faldella, “Pharmacological therapy of gastroesophageal reflux in preterm infants,” Gastroenterology Research and Practice, vol. 2013, Article ID 714564, 12 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  132. F. C. Hampson, A. Farndale, V. Strugala, J. Sykes, I. G. Jolliffe, and P. W. Dettmar, “Alginate rafts and their characterisation,” International Journal of Pharmaceutics, vol. 294, no. 1-2, pp. 137–147, 2005. View at Publisher · View at Google Scholar · View at Scopus
  133. DrugBank Database (Internet), University of Alberta, Canada, http://www.drugbank.ca.
  134. K. G. Mandel, B. P. Daggy, D. A. Brodie, and H. I. Jacoby, “Review article: alginate-raft formulations in the treatment of heartburn and acid reflux,” Alimentary Pharmacology & Therapeutics, vol. 14, no. 6, pp. 669–690, 2000. View at Publisher · View at Google Scholar · View at Scopus
  135. N. Yavorska, “Sodium alginate—a potential tool for weight management: effect on subjective appetite, food intake, and glycemic and insulin regulation,” Journal of Undergraduate Life Sciences, vol. 6, no. 1, pp. 66–69, 2012. View at Google Scholar
  136. J. A. Williams, C. S. Lai, H. Corwin et al., “Inclusion of guar gum and alginate into a crispy bar improves postprandial glycemia in human,” The Journal of Nutrition, vol. 134, no. 4, pp. 886–889, 2004. View at Google Scholar · View at Scopus
  137. B. W. Wolf, C.-S. Lai, M. S. Kipnes et al., “Glycemic and insulinemic responses of nondiabetic healthy adult subjects to an experimental acid-induced viscosity complex incorporated into a glucose beverage,” Nutrition, vol. 18, no. 7-8, pp. 621–626, 2002. View at Publisher · View at Google Scholar · View at Scopus
  138. I. Torsdottir, M. Alpsten, G. Holm, A.-S. Sandberg, and J. Tolli, “A small dose of soluble alginate-fiber affects postprandial glycemia and gastric emptying in humans with diabetes,” The Journal of Nutrition, vol. 121, no. 6, pp. 795–799, 1991. View at Google Scholar · View at Scopus
  139. C. A. Tarling, K. Woods, R. Zhang et al., “The search for novel human pancreatic α-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts,” ChemBioChem, vol. 9, no. 3, pp. 433–438, 2008. View at Publisher · View at Google Scholar · View at Scopus
  140. Y. Idota, Y. Kogure, T. Kato et al., “Cholesterol-lowering effect of calcium alginate in rats,” Biological & Pharmaceutical Bulletin, vol. 39, no. 1, pp. 62–67, 2016. View at Publisher · View at Google Scholar · View at Scopus
  141. J. R. Paxman, J. C. Richardson, P. W. Dettmar, and B. M. Corfe, “Alginate reduces the increased uptake of cholesterol and glucose in overweight male subjects: a pilot study,” Nutrition Research, vol. 28, no. 8, pp. 501–505, 2008. View at Publisher · View at Google Scholar · View at Scopus
  142. Y. Kimura, K. Watanabe, and H. Okuda, “Effects of soluble sodium alginate on cholesterol excretion and glucose tolerance in rats,” Journal of Ethnopharmacology, vol. 54, no. 1, pp. 47–54, 1996. View at Publisher · View at Google Scholar · View at Scopus
  143. M. Georg Jensen, C. Pedersen, M. Kristensen, G. Frost, and A. Astrup, “Review: efficacy of alginate supplementation in relation to appetite regulation and metabolic risk factors: evidence from animal and human studies,” Obesity Reviews, vol. 14, no. 2, pp. 129–144, 2013. View at Publisher · View at Google Scholar · View at Scopus
  144. D. Houghton, M. D. Wilcox, P. I. Chater, I. A. Brownlee, C. J. Seal, and J. P. Pearson, “Biological activity of alginate and its effect on pancreatic lipase inhibition as a potential treatment for obesity,” Food Hydrocolloids, vol. 49, pp. 18–24, 2015. View at Publisher · View at Google Scholar · View at Scopus
  145. D.-H. Ngo and S.-K. Kim, “Sulfated polysaccharides as bioactive agents from marine algae,” International Journal of Biological Macromolecules, vol. 62, pp. 70–75, 2013. View at Publisher · View at Google Scholar · View at Scopus
  146. J.-B. Lee, A. Takeshita, K. Hayashi, and T. Hayashi, “Structures and antiviral activities of polysaccharides from Sargassum trichophyllum,” Carbohydrate Polymers, vol. 86, no. 2, pp. 995–999, 2011. View at Publisher · View at Google Scholar · View at Scopus
  147. G. L. Yan, Y. M. Guo, J. M. Yuan, D. Liu, and B. K. Zhang, “Sodium alginate oligosaccharides from brown algae inhibit Salmonella enteritidis colonization in broiler chickens,” Poultry Science, vol. 90, no. 7, pp. 1441–1448, 2011. View at Publisher · View at Google Scholar · View at Scopus
  148. S. Benavides, R. Villalobos-Carvajal, and J. E. Reyes, “Physical, mechanical and antibacterial properties of alginate film: effect of the crosslinking degree and oregano essential oil concentration,” Journal of Food Engineering, vol. 110, no. 2, pp. 232–239, 2012. View at Publisher · View at Google Scholar · View at Scopus
  149. E.-W. Son, D.-K. Rhee, and S. Pyo, “Antiviral and tumoricidal activities of alginate-stimulated macrophages are mediated by different mechanisms,” Archives of Pharmacal Research, vol. 26, no. 11, pp. 960–966, 2003. View at Publisher · View at Google Scholar · View at Scopus
  150. A. Ahmadi, S. Z. Moghadamtousi, S. Abubakar, and K. Zandi, “Antiviral potential of algae polysaccharides isolated from marine sources: a review,” BioMed Research International, vol. 2015, Article ID 825203, 10 pages, 2015. View at Publisher · View at Google Scholar · View at Scopus
  151. S.-X. Wang, X.-S. Zhang, H.-S. Guan, and W. Wang, “Potential anti-HPV and related cancer agents from marine resources: an overview,” Marine Drugs, vol. 12, no. 4, pp. 2019–2035, 2014. View at Publisher · View at Google Scholar · View at Scopus
  152. Y. Sano, “Antiviral activity of alginate against infection by tobacco mosaic virus,” Carbohydrate Polymers, vol. 38, no. 2, pp. 183–186, 1999. View at Publisher · View at Google Scholar · View at Scopus
  153. M. Witvrouw and E. De Clercq, “Sulfated polysaccharides extracted from sea algae as potential antiviral drugs,” General Pharmacology, vol. 29, no. 4, pp. 497–511, 1997. View at Publisher · View at Google Scholar · View at Scopus
  154. G. Meiyu, L. Fuchuan, X. Xianliang, L. Jing, Y. Zuowei, and G. Huashi, “The potential molecular targets of marine sulfated polymannuroguluronate interfering with HIV-1 entry. Interaction between SPMG and HIV-1 rgp120 and CD4 molecule,” Antiviral Research, vol. 59, no. 2, pp. 127–135, 2003. View at Publisher · View at Google Scholar · View at Scopus
  155. E. H. Son, E. Y. Moon, D. K. Rhee, and S. Pyo, “Stimulation of various functions in murine peritoneal macrophages by high mannuronic acid–containing alginate (HMA) exposure in vivo,” International Immunopharmacology, vol. 1, no. 1, pp. 147–154, 2001. View at Publisher · View at Google Scholar · View at Scopus
  156. Z.-A. Yao, H.-G. Wu, B.-Q. Han, H.-M. Ma, Z.-F. Jiang, and Y.-G. Du, “The antithrombotic action of propylene glycol mannite sulfate (PGMS),” Pharmacological Research, vol. 53, no. 2, pp. 166–170, 2006. View at Publisher · View at Google Scholar · View at Scopus
  157. A. K. Taşkın, M. Yaşar, I. Ozaydın et al., “The hemostatic effect of calcium alginate in experimental splenic injury model,” Turkish Journal of Trauma & Emergency Surgery, vol. 19, no. 3, pp. 195–199, 2013. View at Publisher · View at Google Scholar
  158. H. Hattori, Y. Amano, Y. Nogami, B. Takase, and M. Ishihara, “Hemostasis for severe hemorrhage with photocrosslinkable chitosan hydrogel and calcium alginate,” Annals of Biomedical Engineering, vol. 38, no. 12, pp. 3724–3732, 2010. View at Publisher · View at Google Scholar · View at Scopus
  159. K. Kaneda, S. Kuroda, N. Goto, D. Sato, K. I. Ohya, and S. Kasugai, “Is sodium alginate an alternative hemostatic material in the tooth extraction socket?” Journal of Oral Tissue Engineering, vol. 5, no. 3, pp. 127–133, 2007. View at Publisher · View at Google Scholar
  160. Y. Zeng, D. Yang, P. Qiu et al., “Efficacy of Heparinoid PSS in treating cardiovascular diseases and beyond—a review of 27 years clinical experiences in China,” Clinical and Applied Thrombosis/Hemostasis, vol. 22, no. 3, pp. 222–229, 2016. View at Publisher · View at Google Scholar · View at Scopus
  161. Y.-Y. Chen, W. Ji, J.-R. Du et al., “Preventive effects of low molecular mass potassium alginate extracted from brown algae on DOCA salt-induced hypertension in rats,” Biomedicine & Pharmacotherapy, vol. 64, no. 4, pp. 291–295, 2010. View at Publisher · View at Google Scholar · View at Scopus
  162. M. C. Rocha De Souza, C. T. Marques, C. M. Guerra Dore, F. R. Ferreira Da Silva, H. A. Oliveira Rocha, and E. L. Leite, “Antioxidant activities of sulfated polysaccharides from brown and red seaweeds,” Journal of Applied Phycology, vol. 19, no. 2, pp. 153–160, 2007. View at Publisher · View at Google Scholar · View at Scopus
  163. F. Namvar, R. Mohamad, J. Baharara, S. Zafar-Balanejad, F. Fargahi, and H. S. Rahman, “Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum),” BioMed Research International, vol. 2013, Article ID 604787, 9 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  164. Y. Yamamoto, M. Kurachi, K. Yamaguchi, and T. Oda, “Stimulation of multiple cytokine production in mice by alginate oligosaccharides following intraperitoneal administration,” Carbohydrate Research, vol. 342, no. 8, pp. 1133–1137, 2007. View at Publisher · View at Google Scholar · View at Scopus
  165. T. I. Imbs, S. P. Ermakova, O. S. Malyarenko (Vishchuk), V. V. Isakov, and T. N. Zvyagintseva, “Structural elucidation of polysaccharide fractions from the brown alga Coccophora langsdorfii and in vitro investigation of their anticancer activity,” Carbohydrate Polymers, vol. 135, pp. 162–168, 2016. View at Publisher · View at Google Scholar · View at Scopus
  166. D. Maciel, P. Figueira, S. Xiao et al., “Redox-responsive alginate nanogels with enhanced anticancer cytotoxicity,” Biomacromolecules, vol. 14, no. 9, pp. 3140–3146, 2013. View at Publisher · View at Google Scholar · View at Scopus
  167. H.-J. Jeong, S.-A. Lee, P.-D. Moon et al., “Alginic acid has anti-anaphylactic effects and inhibits inflammatory cytokine expression via suppression of nuclear factor-κB activation,” Clinical and Experimental Allergy, vol. 36, no. 6, pp. 785–794, 2006. View at Publisher · View at Google Scholar · View at Scopus
  168. T. Uno, M. Hattori, and T. Yoshida, “Oral administration of alginic acid oligosaccharide suppresses IgE production and inhibits the induction of oral tolerance,” Bioscience, Biotechnology and Biochemistry, vol. 70, no. 12, pp. 3054–3057, 2006. View at Publisher · View at Google Scholar · View at Scopus
  169. A. B. A. Ahmed, M. Adel, P. Karimi, and M. Peidayesh, “Chapter ten—pharmaceutical, cosmeceutical, and traditional applications of marine carbohydrates,” Advances in Food and Nutrition Research, vol. 73, pp. 197–220, 2014. View at Publisher · View at Google Scholar · View at Scopus
  170. T. Andersen, C. Markussen, M. Dornish et al., “In situ gelation for cell immobilization and culture in alginate foam scaffolds,” Tissue Engineering Part A, vol. 20, no. 3-4, pp. 600–610, 2014. View at Publisher · View at Google Scholar · View at Scopus
  171. H. Geckil, F. Xu, X. Zhang, S. Moon, and U. Demirci, “Engineering hydrogels as extracellular matrix mimics,” Nanomedicine, vol. 5, no. 3, pp. 469–484, 2010. View at Publisher · View at Google Scholar · View at Scopus
  172. C. Godugu and M. Singh, “AlgiMatrix™-based 3D cell culture system as an in vitro tumor model: an important tool in cancer research,” in Cancer Chemoprevention, vol. 1379 of Methods in Molecular Biology, pp. 117–128, Springer, 2016. View at Publisher · View at Google Scholar
  173. T. Andersen, P. Auk-Emblem, and M. Dornish, “3D cell culture in alginate hydrogels,” Microarrays, vol. 4, no. 2, pp. 133–161, 2015. View at Publisher · View at Google Scholar
  174. C. Godugu, A. R. Patel, U. Desai, T. Andey, A. Sams, and M. Singh, “AlgiMatrix™ based 3D cell culture system as an in-vitro tumor model for anticancer studies,” PLoS ONE, vol. 8, no. 1, Article ID e53708, 2013. View at Publisher · View at Google Scholar · View at Scopus
  175. S. J. M. Skinner, M. S. Geaney, H. Lin et al., “Encapsulated living choroid plexus cells: potential long-term treatments for central nervous system disease and trauma,” Journal of Neural Engineering, vol. 6, no. 6, Article ID 065001, 2009. View at Publisher · View at Google Scholar · View at Scopus
  176. L. N. Pettingill, A. K. Wise, M. S. Geaney, and R. K. Shepherd, “Enhanced auditory neuron survival following cell-based BDNF treatment in the deaf guinea pig,” PLoS ONE, vol. 6, no. 4, Article ID e18733, 2011. View at Publisher · View at Google Scholar · View at Scopus
  177. H. K. Yang and K.-H. Yoon, “Current status of encapsulated islet transplantation,” Journal of Diabetes and its Complications, vol. 29, no. 5, pp. 737–743, 2015. View at Publisher · View at Google Scholar · View at Scopus
  178. A. L. Hillberg, K. Kathirgamanathan, J. B. Lam, L. Y. Law, O. Garkavenko, and R. B. Elliott, “Improving alginate-poly-L-ornithine-alginate capsule biocompatibility through genipin crosslinking,” Journal of Biomedical Materials Research—Part B Applied Biomaterials, vol. 101, no. 2, pp. 258–268, 2013. View at Publisher · View at Google Scholar · View at Scopus
  179. M. Qi, “Transplantation of encapsulated pancreatic islets as a treatment for patients with type 1 diabetes mellitus,” Advances in Medicine, vol. 2014, Article ID 429710, 15 pages, 2014. View at Publisher · View at Google Scholar
  180. J. Schweicher, C. Nyitray, and T. A. Desai, “Membranes to achieve immunoprotection of transplanted islets,” Frontiers in Bioscience, vol. 19, no. 1, pp. 49–76, 2014. View at Publisher · View at Google Scholar · View at Scopus
  181. Clinical trials (Internet), U.S. National Institutes of Health, https://clinicaltrials.gov/ct2/results?term=alginate&Search=Search.
  182. S. Irving, L. Gillespie, R. Richardson, D. Rowe, J. B. Fallon, and A. K. Wise, “Electroacoustic stimulation: now and into the future,” BioMed Research International, vol. 2014, Article ID 350504, 17 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  183. European Parkinson’s Disease Association (Internet), http://www.epda.eu.com/en/news/news-archive/2016/06-07-lct/.
  184. L. C. Powell, A. Sowedan, S. Khan et al., “The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms,” Biofouling, vol. 29, no. 4, pp. 413–421, 2013. View at Publisher · View at Google Scholar · View at Scopus
  185. W. Hengzhuang, Z. Song, O. Ciofu, E. Onsøyen, P. D. Rye, and N. Høiby, “OligoG CF-5/20 disruption of mucoid Pseudomonas aeruginosa biofilm in a murine lung infection model,” Antimicrobial Agents and Chemotherapy, vol. 60, no. 5, pp. 2620–2626, 2016. View at Publisher · View at Google Scholar
  186. A. Tøndervik, H. Sletta, G. Klinkenberg et al., “Alginate oligosaccharides inhibit fungal cell growth and potentiate the activity of antifungals against Candida and Aspergillus spp.,” PLoS ONE, vol. 9, no. 11, Article ID e112518, 2014. View at Publisher · View at Google Scholar · View at Scopus
  187. S. Chaluvadi, A. T. Hotchkiss Jr., J. E. Call et al., “Protection of probiotic bacteria in a synbiotic matrix following aerobic storage at 4 °C,” Beneficial Microbes, vol. 3, no. 3, pp. 175–187, 2012. View at Publisher · View at Google Scholar · View at Scopus