(1→3)-α-d-Glucan from Fruiting Body and Mycelium of Cerrena unicolor (Bull.) Murrill: Structural Characterization and Use as a Novel Inducer of Mutanase

Table 3

Summary of methylation analysis of glucans extracted from fruiting body and vegetative mycelium of C. unicolor.

Origin	Methylated sugar	Linkage type	Molar amounts (%)
Fruiting body	2,3,4,6-O-Me4-D-glucose	α-D-Glcp-(1	0.2
	2,4,6-O-Me3-D-glucose	3)-α-D-Glcp-(1	92.2
	2,3,6-O-Me3-D-glucose	4)-α-D-Glcp-(1	4.4
	2,6-O-Me2-D-glucose	3,4)-α-D-Glcp-(1	3.2
Mycelium	2,4,6-O-Me3-D-glucose	3)-α-D-Glcp-(1	90.1
	2,3,6-O-Me3-D-glucose	4)-α-D-Glcp-(1	7.4
	2,6-O-Me2-D-glucose	3,4)-α-D-Glcp-(1	2.5

The glucan preparation was methylated before being hydrolysed, reduced, and acetylated. Therefore, the methylated sugars mentioned refer to permethylated alditol acetates identified by GC–MS (e.g., 2,3,4,6-O-Me₄-D-glucose refers to 1,5-di-O-acetyl-2,3,4,6-tetra-O-methyl-D-glucitol).