Thermal Synthesis of Polypeptides from N-Butyloxycarbonyl Oligopeptides Containing Aspartyl Residue at C-Terminus

Figure 11

Calibration curve of the molecular weight of peptides using HPLC with a TSK-GEL G-3000PW (300 mm × 7.5 mm i.d.). Eluent: 0.1 M sodium phosphate buffer, pH 6.9; flow rate: 0.7 mL/min; detection: UV 230 nm. : volume (mL) of elution for each sample; : elution volume of blue dextran as void volume (mL).