Thermal Synthesis of Polypeptides from N-Butyloxycarbonyl Oligopeptides Containing Aspartyl Residue at C-Terminus

Figure 9

Gel permeation chromatograms of the reaction mixtures of peptide derivatives under different conditions. (a) Boc-L-Ala-L-Asp, 130°C, 1 h; (b) Boc-L-Val-L-Asp, 130°C, 2 h; and (c) 120°C, 4 h. Solid phase: Sephadex G-25F. Eluate: 0.5 M acetic acid. Volume per a test tube: 3 mL. fa: ninhydrin-negative, higher molecular weight fractions; fb: ninhydrin-positive lower molecular weight fractions.

(a)

(b)

(c)