Thermal Synthesis of Polypeptides from N-Butyloxycarbonyl Oligopeptides Containing Aspartyl Residue at C-Terminus

Table 2

Estimated molecular weight of the higher molecular weight fraction.

Substrate	Temperature/°C	Reaction time/h		Molecular weight/Da	Number () of residues (AA-Asp)
Boc-Gly-L-Asap (5a)	110	24	1.395	4400	26
	120	24	1.362	7000	41
	130	1	1.423	2800	16
	130	2	1.397	4100	24
	130	4	1.363	6900	40
	130	8	1.354	7800	45
	130	16	1.381	5400	31
	130	24	1.389	4600	23
	150	2	1.430	2500	15
	170	2	1.468	1400	8
Boc-L-Ala-L-Asp (5b)	130	1	1.466	1500	8
	130		1.421	2900	16
	130		1.382	5200	28
Boc-L-Val-L-Asp (5c)	120		1.447	2000	9
	130	2	1.441	2200	10
	130		1.393	4200	20
Boc-Gly-Gly-L-Asp (5d)	120	4	1.354	7800	34
	120		1.404	3700	16
	130		1.402	4000	17
	130		1.385	5000	21

: volume of elution; : void volume; mixture without gel filtration.